Characterization of the equine 2′-5′ oligoadenylate synthetase 1 (OAS1) and ribonuclease L (RNASEL) innate immunity genes

Jonathan J. Rios, Andrey A. Perelygin, Maureen T. Long, Teri L. Lear, Andrey A. Zharkikh, Margo A. Brinton, David L. Adelson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: The mammalian OAS/RNASEL pathway plays an important role in antiviral host defense. A premature stop-codon within the murine Oas1b gene results in the increased susceptibility of mice to a number of flaviviruses, including West Nile virus (WNV). Mutations in either the OAS1 or RNASEL genes may also modulate the outcome of WNV-induced disease or other viral infections in horses. Polymorphisms in the human OAS gene cluster have been previously utilized for case-control analysis of virus-induced disease in humans. No polymorphisms have yet been identified in either the equine OAS1 or RNASEL genes for use in similar case-control studies. Results: Genomic sequence for equine OAS1 was obtained from a contig assembly generated from a shotgun subclone library of CHORI-241 BAC 100I10. Specific amplification of regions of the OAS1 gene from 13 horses of various breeds identified 33 single nucleotide polymorphisms (SNP) and two microsatellites. RNASEL cDNA sequences were determined for 8 mammals and utilized in a phylogenetic analysis. The chromosomal location of the RNASEL gene was assigned by FISH to ECA5p17-p16 using two selected CHORI-241 BAC clones. The horse genomic RNASEL sequence was assembled. Specific amplification of regions of the RNASEL gene from 13 horses identified 31 SNPs. Conclusion: In this report, two dinucleotide microsatellites and 64 single nucleotide polymorphisms within the equine OAS1 and RNASEL genes were identified. These polymorphisms are the first to be reported for these genes and will facilitate future case-control studies of horse susceptibility to infectious diseases.

Original languageEnglish (US)
Article number313
JournalBMC Genomics
Volume8
DOIs
StatePublished - Sep 7 2007

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Ligases
Ribonucleases
Innate Immunity
Horses
Genes
Virus Diseases
Single Nucleotide Polymorphism
West Nile virus
Microsatellite Repeats
Case-Control Studies
Flavivirus
2',5'-oligoadenylate
Nonsense Codon
Firearms
Multigene Family
Libraries
Antiviral Agents
Communicable Diseases
Mammals
Complementary DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Characterization of the equine 2′-5′ oligoadenylate synthetase 1 (OAS1) and ribonuclease L (RNASEL) innate immunity genes. / Rios, Jonathan J.; Perelygin, Andrey A.; Long, Maureen T.; Lear, Teri L.; Zharkikh, Andrey A.; Brinton, Margo A.; Adelson, David L.

In: BMC Genomics, Vol. 8, 313, 07.09.2007.

Research output: Contribution to journalArticle

Rios, Jonathan J. ; Perelygin, Andrey A. ; Long, Maureen T. ; Lear, Teri L. ; Zharkikh, Andrey A. ; Brinton, Margo A. ; Adelson, David L. / Characterization of the equine 2′-5′ oligoadenylate synthetase 1 (OAS1) and ribonuclease L (RNASEL) innate immunity genes. In: BMC Genomics. 2007 ; Vol. 8.
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abstract = "Background: The mammalian OAS/RNASEL pathway plays an important role in antiviral host defense. A premature stop-codon within the murine Oas1b gene results in the increased susceptibility of mice to a number of flaviviruses, including West Nile virus (WNV). Mutations in either the OAS1 or RNASEL genes may also modulate the outcome of WNV-induced disease or other viral infections in horses. Polymorphisms in the human OAS gene cluster have been previously utilized for case-control analysis of virus-induced disease in humans. No polymorphisms have yet been identified in either the equine OAS1 or RNASEL genes for use in similar case-control studies. Results: Genomic sequence for equine OAS1 was obtained from a contig assembly generated from a shotgun subclone library of CHORI-241 BAC 100I10. Specific amplification of regions of the OAS1 gene from 13 horses of various breeds identified 33 single nucleotide polymorphisms (SNP) and two microsatellites. RNASEL cDNA sequences were determined for 8 mammals and utilized in a phylogenetic analysis. The chromosomal location of the RNASEL gene was assigned by FISH to ECA5p17-p16 using two selected CHORI-241 BAC clones. The horse genomic RNASEL sequence was assembled. Specific amplification of regions of the RNASEL gene from 13 horses identified 31 SNPs. Conclusion: In this report, two dinucleotide microsatellites and 64 single nucleotide polymorphisms within the equine OAS1 and RNASEL genes were identified. These polymorphisms are the first to be reported for these genes and will facilitate future case-control studies of horse susceptibility to infectious diseases.",
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