Characterization of the larynx in ephrin-B2 knockout mice: A novel animal model for laryngeal clefts

Ryan E. Neilan, Dongmei Shao, Christopher Dravis, Mark Henkemeyer, Kenneth H. Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Objective: To identify and classify laryngeal clefts in a novel mouse model. Design: In vivo animal study. Setting: Academic research laboratory. Subjects: 129/CD1 mice with the ephrin-B2 gene disrupted by the β-galactosidase ( lacZ ) gene were humanely killed at embryonic day 18 (E18) and evaluated for the presence and characterization of a laryngeal cleft. Homozygous and heterozygous lacZ knockout mice as well as wild-type littermates were evaluated. Main Outcome Measures: Microsurgical dissection of the oral cavity and pharynx allowed for a pseudoendoscopic view of the larynx to determine the presence or absence of a cleft. The specimens were also histologically sectioned and examined for characterization and classification of the cleft. Results: A laryngeal cleft was identified in 12 of 27 ephrin-B2 homozygous lacZ knockout mice (44%). Laryngeal clefts were not identified in heterozygous ephrin-B2 knockout mice or in wild-type littermates. Conclusions: Disruption of ephrin-B2 reverse signaling results in laryngeal clefts in lacZ knockout mice. This presents a novel mouse model in which future investigations into etiology of laryngeal clefts may be examined.

Original languageEnglish (US)
Pages (from-to)969-972
Number of pages4
JournalArchives of Otolaryngology - Head and Neck Surgery
Issue number10
StatePublished - Oct 2012

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology


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