TY - JOUR
T1 - Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes
AU - Sperandio, Vanessa
AU - Kaper, James B.
AU - Bortolini, Mafalda Regina
AU - Cruz Neves, Bianca
AU - Keller, Rogeria
AU - Trabulsi, Luiz R.
N1 - Funding Information:
We thank Dr. Tania Tardelli Gomes from Escola Paulista de Medicina, São Paulo, Brazil for the gift of some radioactive isotopes used in this study and Dr. Timothy McDaniel, Stanford University for the LEE probes. This work was supported by grants FAPESP 96/4148-5, FINEP/MCT/PRONEX 41.96.0881.00 and National Institutes of Health AI 21657.
PY - 1998/7/1
Y1 - 1998/7/1
N2 - All proteins involved in the attachment and effacement lesion produced by enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing E. coli (STEC) are encoded by the locus of enterocyte effacement (LEE). We studied the presence and insertion site of the LEE in different EPEC and STEC strains. In serotypes O119:H6/H-, O55:H6, O55:H7, O142:H6, O111ac:H9/H-, O111ab:H9/H- LEE is inserted downstream of selC as previously described for EPEC O127:H6 and STEC O157:H7. In serotypes O111ac:H8/H- and O26:H11/H- the LEE is inserted in pheU as previously described for STEC O26:H-. However in EPEC from serotype O111ab:H25 the LEE is not inserted in either site suggesting a third insertion site in the K12 chromosome. We also cloned fragments of 2.3 kb and 1.0 kb from the right and left hand sides of the LEE of a O111ac:H- strain and identified additional insertion sequences on these LEE fragments, suggesting that the LEE may be larger and may have undergone more recombination events in these serotypes.
AB - All proteins involved in the attachment and effacement lesion produced by enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing E. coli (STEC) are encoded by the locus of enterocyte effacement (LEE). We studied the presence and insertion site of the LEE in different EPEC and STEC strains. In serotypes O119:H6/H-, O55:H6, O55:H7, O142:H6, O111ac:H9/H-, O111ab:H9/H- LEE is inserted downstream of selC as previously described for EPEC O127:H6 and STEC O157:H7. In serotypes O111ac:H8/H- and O26:H11/H- the LEE is inserted in pheU as previously described for STEC O26:H-. However in EPEC from serotype O111ab:H25 the LEE is not inserted in either site suggesting a third insertion site in the K12 chromosome. We also cloned fragments of 2.3 kb and 1.0 kb from the right and left hand sides of the LEE of a O111ac:H- strain and identified additional insertion sequences on these LEE fragments, suggesting that the LEE may be larger and may have undergone more recombination events in these serotypes.
KW - Attachment and effacement
KW - Enteropatogenic Escherichia coli
KW - Pathogenicity island
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U2 - 10.1016/S0378-1097(98)00205-5
DO - 10.1016/S0378-1097(98)00205-5
M3 - Article
C2 - 9675859
AN - SCOPUS:0032125254
SN - 0378-1097
VL - 164
SP - 133
EP - 139
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -