Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes

Vanessa Sperandio, James B. Kaper, Mafalda Regina Bortolini, Bianca Cruz Neves, Rogeria Keller, Luiz R. Trabulsi

Research output: Contribution to journalArticle

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Abstract

All proteins involved in the attachment and effacement lesion produced by enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing E. coli (STEC) are encoded by the locus of enterocyte effacement (LEE). We studied the presence and insertion site of the LEE in different EPEC and STEC strains. In serotypes O119:H6/H-, O55:H6, O55:H7, O142:H6, O111ac:H9/H-, O111ab:H9/H- LEE is inserted downstream of selC as previously described for EPEC O127:H6 and STEC O157:H7. In serotypes O111ac:H8/H- and O26:H11/H- the LEE is inserted in pheU as previously described for STEC O26:H-. However in EPEC from serotype O111ab:H25 the LEE is not inserted in either site suggesting a third insertion site in the K12 chromosome. We also cloned fragments of 2.3 kb and 1.0 kb from the right and left hand sides of the LEE of a O111ac:H- strain and identified additional insertion sequences on these LEE fragments, suggesting that the LEE may be larger and may have undergone more recombination events in these serotypes.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalFEMS Microbiology Letters
Volume164
Issue number1
DOIs
StatePublished - Jul 1 1998

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Shiga-Toxigenic Escherichia coli
Enteropathogenic Escherichia coli
Enterocytes
Escherichia coli O157
Serogroup
Insertional Mutagenesis
Genetic Recombination
Chromosomes

Keywords

  • Attachment and effacement
  • Enteropatogenic Escherichia coli
  • Pathogenicity island

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes. / Sperandio, Vanessa; Kaper, James B.; Bortolini, Mafalda Regina; Cruz Neves, Bianca; Keller, Rogeria; Trabulsi, Luiz R.

In: FEMS Microbiology Letters, Vol. 164, No. 1, 01.07.1998, p. 133-139.

Research output: Contribution to journalArticle

Sperandio, Vanessa ; Kaper, James B. ; Bortolini, Mafalda Regina ; Cruz Neves, Bianca ; Keller, Rogeria ; Trabulsi, Luiz R. / Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes. In: FEMS Microbiology Letters. 1998 ; Vol. 164, No. 1. pp. 133-139.
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