Characterization of the regulation of renal Na+/H+ exchanger NHE3 by insulin

Daniel G. Fuster, I. Alexandru Bobulescu, Jianning Zhang, James Wade, Orson W. Moe

Research output: Contribution to journalArticle

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Abstract

Insulin receptors are widely distributed in the kidney and affect multiple aspects of renal function. In the proximal tubule, insulin regulates volume and acid-base regulation through stimulation of the Na+/H+ exchanger NHE3. This paper characterizes the signaling pathway by which insulin stimulates NHE3 in a cell culture model [opossum kidney (OK) cell]. Insulin has two distinct phases of action on NHE3. Chronic insulin (24 h) activates NHE3 through the classic phosphatidylinositol 3-kinase-serum- and glucocorticoid-dependent kinase 1 (PI3K-SGK1) pathway as insulin stimulates SGK1 phosphorylation and the insulin effect can be blocked by the PI3K inhibitor wortmannin or a dominant-negative SGK1. We showed that SGK1 transcript and protein are expressed in rat proximal tubule and OK cells. We previously showed that glucocorticoids augment the effect of insulin on NHE3 (Klisic J, Hu MC, Nief V, Reyes L, Fuster D, Moe OW, Ambuhl PM. Am J Physiol Renal Physiol 283: F532-F539, 2002). Part of this can be mediated via induction of SGK1 by glucocorticoids, and indeed the insulin effect on NHE3 can also be amplified by overexpression of SGK1. We next addressed the acute effect of insulin (1-2 h) on NHE3 by systematically examining the candidate signaling cascades and activation mechanisms of NHE3. We ruled out the PI3K-SGK1-Akt and TC10 pathways, increased surface NHE3, NHE3 phosphorylation, NHE3 association with calcineurin homologous protein 1 or megalin as mechanisms of acute activation of NHE3 by insulin. In summary, insulin stimulates NHE3 acutely via yet undefined pathways and mechanisms. The chronic effect of insulin is mediated by the classic PI3K-SGK1 route.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume292
Issue number2
DOIs
StatePublished - Feb 2007

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Sodium-Hydrogen Antiporter
Insulin
Kidney
Glucocorticoids
Phosphatidylinositol 3-Kinase
Opossums
Phosphotransferases
Low Density Lipoprotein Receptor-Related Protein-2
Serum
Phosphorylation
Proximal Kidney Tubule
Calcineurin
Insulin Receptor
Phosphatidylinositol 3-Kinases
Proteins
Cell Culture Techniques

Keywords

  • Na/H exchanger isoform 3
  • Serum- and glucocorticoid-dependent kinase 1

ASJC Scopus subject areas

  • Physiology

Cite this

Characterization of the regulation of renal Na+/H+ exchanger NHE3 by insulin. / Fuster, Daniel G.; Bobulescu, I. Alexandru; Zhang, Jianning; Wade, James; Moe, Orson W.

In: American Journal of Physiology - Renal Physiology, Vol. 292, No. 2, 02.2007.

Research output: Contribution to journalArticle

Fuster, Daniel G. ; Bobulescu, I. Alexandru ; Zhang, Jianning ; Wade, James ; Moe, Orson W. / Characterization of the regulation of renal Na+/H+ exchanger NHE3 by insulin. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 292, No. 2.
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