Characterization of the role of DR0171 in transcriptional response to radiation in the extremely radioresistant bacterium Deinococcus radiodurans

Huiming Lu, Wenrong Xia, Huan Chen, Longfei Yin, Xiaojun Zhao, Guangzhi Xu, Yuejin Hua

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The extremely radioresistant bacterium Deinococcus radiodurans encodes a number of function-unknown genes, and some of them involve in the radioresistance. The radiation-inducible gene dr0171 has a recA-like expression pattern in the postirradiation recovery and was also supposed to encode a transcriptional regulator to contribute to the radioresistance. Here, we found that the EGFP-tagged DR0171 proteins gathered in the nucleoid regions after radiation. Further, we constructed a null mutant of dr0171 and found that the incapacitation of the dr0171 led to a signiWcant decline in resistance to -rays, UV radiation, and hydrogen peroxide and delayed genomic DNA reconstruction after radiation, indicating that this gene is involved in the postirradiation recovery. The microarray assays showed that the disruption of dr0171 does not lead to a signiWcant change in the transcriptional proWle of D. radiodurans under normal conditions, except after the stress of 6 kGy, radiation was applied. The transcript level of at least 153 genes decreased over twofold in the irradiated dr0171 mutant compared with those in the irradiated wild-type strain, indicating that DR0171 only functions after radiation damage. Taken together, the data presented here indicate that DR0171 serves as a regulator of the transcriptional response to radiation damage in D. radiodurans.

Original languageEnglish (US)
Pages (from-to)741-750
Number of pages10
JournalArchives of Microbiology
Volume193
Issue number10
DOIs
StatePublished - Oct 1 2011

Keywords

  • DR0171
  • Deinococcus radiodurans
  • Microarray
  • Radiation resistance
  • Response regulator

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Characterization of the role of DR0171 in transcriptional response to radiation in the extremely radioresistant bacterium Deinococcus radiodurans'. Together they form a unique fingerprint.

  • Cite this