TY - JOUR
T1 - Characterization of the role of DR0171 in transcriptional response to radiation in the extremely radioresistant bacterium Deinococcus radiodurans
AU - Lu, Huiming
AU - Xia, Wenrong
AU - Chen, Huan
AU - Yin, Longfei
AU - Zhao, Xiaojun
AU - Xu, Guangzhi
AU - Hua, Yuejin
N1 - Funding Information:
Acknowledgments This work was supported by grants from National Natural Science Foundation of China (30830006, 31000045), Special Fund for AgroscientiWc Research in the Public Interest (201103007), a major scientiWc and technological project for signiWcant new drugs creation (2009ZXJ09001-034), a major project for genetically modiWed organisms breeding (2009ZX08009-075B), grants from China Postdoctoral Science Foundation (20090460101, 201003728), and a grant from Zhejiang Wanli University (KF2010003). We thank Professor Junjie Fu and Zhiming Sun (Radiation Center of Zhejiang University) for their assistance in radiation treatment.
PY - 2011/10
Y1 - 2011/10
N2 - The extremely radioresistant bacterium Deinococcus radiodurans encodes a number of function-unknown genes, and some of them involve in the radioresistance. The radiation-inducible gene dr0171 has a recA-like expression pattern in the postirradiation recovery and was also supposed to encode a transcriptional regulator to contribute to the radioresistance. Here, we found that the EGFP-tagged DR0171 proteins gathered in the nucleoid regions after radiation. Further, we constructed a null mutant of dr0171 and found that the incapacitation of the dr0171 led to a signiWcant decline in resistance to -rays, UV radiation, and hydrogen peroxide and delayed genomic DNA reconstruction after radiation, indicating that this gene is involved in the postirradiation recovery. The microarray assays showed that the disruption of dr0171 does not lead to a signiWcant change in the transcriptional proWle of D. radiodurans under normal conditions, except after the stress of 6 kGy, radiation was applied. The transcript level of at least 153 genes decreased over twofold in the irradiated dr0171 mutant compared with those in the irradiated wild-type strain, indicating that DR0171 only functions after radiation damage. Taken together, the data presented here indicate that DR0171 serves as a regulator of the transcriptional response to radiation damage in D. radiodurans.
AB - The extremely radioresistant bacterium Deinococcus radiodurans encodes a number of function-unknown genes, and some of them involve in the radioresistance. The radiation-inducible gene dr0171 has a recA-like expression pattern in the postirradiation recovery and was also supposed to encode a transcriptional regulator to contribute to the radioresistance. Here, we found that the EGFP-tagged DR0171 proteins gathered in the nucleoid regions after radiation. Further, we constructed a null mutant of dr0171 and found that the incapacitation of the dr0171 led to a signiWcant decline in resistance to -rays, UV radiation, and hydrogen peroxide and delayed genomic DNA reconstruction after radiation, indicating that this gene is involved in the postirradiation recovery. The microarray assays showed that the disruption of dr0171 does not lead to a signiWcant change in the transcriptional proWle of D. radiodurans under normal conditions, except after the stress of 6 kGy, radiation was applied. The transcript level of at least 153 genes decreased over twofold in the irradiated dr0171 mutant compared with those in the irradiated wild-type strain, indicating that DR0171 only functions after radiation damage. Taken together, the data presented here indicate that DR0171 serves as a regulator of the transcriptional response to radiation damage in D. radiodurans.
KW - DR0171
KW - Deinococcus radiodurans
KW - Microarray
KW - Radiation resistance
KW - Response regulator
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U2 - 10.1007/s00203-011-0712-y
DO - 10.1007/s00203-011-0712-y
M3 - Article
C2 - 21567175
AN - SCOPUS:82455209069
SN - 0302-8933
VL - 193
SP - 741
EP - 750
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 10
ER -