Characterization of the spermidine synthase-related gene family in Arabidopsis thaliana

Yoshie Hanzawa, Akihiro Imai, Anthony J. Michael, Yoshibumi Komeda, Taku Takahashi

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The Arabidopsis genome contains four genes that encode proteins similar to both spermidine synthase and spermine synthase of other organisms. Our previous study revealed that one of these genes, designated ACAULIS5 (ACL5), encodes spermine synthase and that its null mutation results in a severe defect in the elongation of stem internodes. Here we report the characterization of the other three genes, designated SPDS1, SPDS2 and SPDS3. Our results showed that SPDS1 and SPDS2 possess spermidine synthase activity in yeast spermidine synthase-deficient mutants, but the enzyme activity of SPDS3 remained to be determined. RNA gel blot analysis revealed that all of these genes are expressed in all plant organs but show different responses to exogenous plant hormones, suggesting that they are involved in different aspects of growth by modulating the contents of polyamines in plant cells.

Original languageEnglish (US)
Pages (from-to)176-180
Number of pages5
JournalFEBS Letters
Volume527
Issue number1-3
DOIs
StatePublished - Sep 11 2002

Fingerprint

Spermidine Synthase
Spermine Synthase
Arabidopsis
Genes
Plant Growth Regulators
Polyamines
Plant Cells
Yeasts
Gels
Genome
RNA
Enzyme activity
Mutation
Yeast
Enzymes
Growth
Elongation
Proteins
Defects

Keywords

  • Arabidopsis thaliana
  • Gene expression
  • Polyamine
  • Spermidine synthase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of the spermidine synthase-related gene family in Arabidopsis thaliana. / Hanzawa, Yoshie; Imai, Akihiro; Michael, Anthony J.; Komeda, Yoshibumi; Takahashi, Taku.

In: FEBS Letters, Vol. 527, No. 1-3, 11.09.2002, p. 176-180.

Research output: Contribution to journalArticle

Hanzawa, Yoshie ; Imai, Akihiro ; Michael, Anthony J. ; Komeda, Yoshibumi ; Takahashi, Taku. / Characterization of the spermidine synthase-related gene family in Arabidopsis thaliana. In: FEBS Letters. 2002 ; Vol. 527, No. 1-3. pp. 176-180.
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