TY - JOUR
T1 - Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63
AU - Habe, Hiroshi
AU - Chung, Jin Sung
AU - Kato, Hiroyuki
AU - Ayabe, Yuko
AU - Kasuga, Kano
AU - Yoshida, Takako
AU - Nojiri, Hideaki
AU - Yamane, Hisakazu
AU - Omori, Toshio
PY - 2004/9
Y1 - 2004/9
N2 - Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1- hydro-9-fluorenone to produce 9-fluorenone and 2′-carboxy-2,3- dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.
AB - Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1- hydro-9-fluorenone to produce 9-fluorenone and 2′-carboxy-2,3- dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.
UR - http://www.scopus.com/inward/record.url?scp=4344610106&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4344610106&partnerID=8YFLogxK
U2 - 10.1128/JB.186.17.5938-5944.2004
DO - 10.1128/JB.186.17.5938-5944.2004
M3 - Article
C2 - 15317800
AN - SCOPUS:4344610106
SN - 0021-9193
VL - 186
SP - 5938
EP - 5944
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 17
ER -