Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63

Hiroshi Habe, Jin Sung Chung, Hiroyuki Kato, Yuko Ayabe, Kano Kasuga, Takako Yoshida, Hideaki Nojiri, Hisakazu Yamane, Toshio Omori

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25 Scopus citations

Abstract

Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1- hydro-9-fluorenone to produce 9-fluorenone and 2′-carboxy-2,3- dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.

Original languageEnglish (US)
Pages (from-to)5938-5944
Number of pages7
JournalJournal of bacteriology
Volume186
Issue number17
DOIs
StatePublished - Sep 1 2004

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Habe, H., Chung, J. S., Kato, H., Ayabe, Y., Kasuga, K., Yoshida, T., Nojiri, H., Yamane, H., & Omori, T. (2004). Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63. Journal of bacteriology, 186(17), 5938-5944. https://doi.org/10.1128/JB.186.17.5938-5944.2004