Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63

Hiroshi Habe, Jin Sung Chung, Hiroyuki Kato, Yuko Ayabe, Kano Kasuga, Takako Yoshida, Hideaki Nojiri, Hisakazu Yamane, Toshio Omori

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1- hydro-9-fluorenone to produce 9-fluorenone and 2′-carboxy-2,3- dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.

Original languageEnglish (US)
Pages (from-to)5938-5944
Number of pages7
JournalJournal of Bacteriology
Volume186
Issue number17
DOIs
StatePublished - Sep 2004

Fingerprint

Genes
Hydrolases
Serine
Proteins
9-fluorenone
fluorene
phthalic acid
extradiol dioxygenase
2,3-dihydroxybiphenyl

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Habe, H., Chung, J. S., Kato, H., Ayabe, Y., Kasuga, K., Yoshida, T., ... Omori, T. (2004). Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63. Journal of Bacteriology, 186(17), 5938-5944. https://doi.org/10.1128/JB.186.17.5938-5944.2004

Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63. / Habe, Hiroshi; Chung, Jin Sung; Kato, Hiroyuki; Ayabe, Yuko; Kasuga, Kano; Yoshida, Takako; Nojiri, Hideaki; Yamane, Hisakazu; Omori, Toshio.

In: Journal of Bacteriology, Vol. 186, No. 17, 09.2004, p. 5938-5944.

Research output: Contribution to journalArticle

Habe, H, Chung, JS, Kato, H, Ayabe, Y, Kasuga, K, Yoshida, T, Nojiri, H, Yamane, H & Omori, T 2004, 'Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63', Journal of Bacteriology, vol. 186, no. 17, pp. 5938-5944. https://doi.org/10.1128/JB.186.17.5938-5944.2004
Habe, Hiroshi ; Chung, Jin Sung ; Kato, Hiroyuki ; Ayabe, Yuko ; Kasuga, Kano ; Yoshida, Takako ; Nojiri, Hideaki ; Yamane, Hisakazu ; Omori, Toshio. / Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 17. pp. 5938-5944.
@article{12c6ac7c34b14df99466f6a42730828a,
title = "Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63",
abstract = "Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1- hydro-9-fluorenone to produce 9-fluorenone and 2′-carboxy-2,3- dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.",
author = "Hiroshi Habe and Chung, {Jin Sung} and Hiroyuki Kato and Yuko Ayabe and Kano Kasuga and Takako Yoshida and Hideaki Nojiri and Hisakazu Yamane and Toshio Omori",
year = "2004",
month = "9",
doi = "10.1128/JB.186.17.5938-5944.2004",
language = "English (US)",
volume = "186",
pages = "5938--5944",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "17",

}

TY - JOUR

T1 - Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63

AU - Habe, Hiroshi

AU - Chung, Jin Sung

AU - Kato, Hiroyuki

AU - Ayabe, Yuko

AU - Kasuga, Kano

AU - Yoshida, Takako

AU - Nojiri, Hideaki

AU - Yamane, Hisakazu

AU - Omori, Toshio

PY - 2004/9

Y1 - 2004/9

N2 - Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1- hydro-9-fluorenone to produce 9-fluorenone and 2′-carboxy-2,3- dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.

AB - Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1- hydro-9-fluorenone to produce 9-fluorenone and 2′-carboxy-2,3- dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.

UR - http://www.scopus.com/inward/record.url?scp=4344610106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344610106&partnerID=8YFLogxK

U2 - 10.1128/JB.186.17.5938-5944.2004

DO - 10.1128/JB.186.17.5938-5944.2004

M3 - Article

VL - 186

SP - 5938

EP - 5944

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 17

ER -