TY - JOUR
T1 - Characterization of two isozymic forms of heart fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase
AU - Sakata, Junichiro
AU - Uyeda, Kosaku
PY - 1991/10/31
Y1 - 1991/10/31
N2 - Occurrence of two isozymic forms of fructose 6-P,2-kinase: fructose 2,6-bisphosphatase in bovine heart was investigated by transcribing mRNAs and amplifying the cDNAs with polymerase chain reactions. Analysis of the PCR products revealed 1.7 Kb and 1.5 Kb DNAs, and the determination of their nucleotide sequences showed that these DNAs are identical except for the lack of 180 base pairs near the 3′ of the bovine heart enzyme DNA previously reported (6). This missing nucleotide sequence encodes Asn451-Gln510 and contains the phosphorylation sites for cAMP dependent protein kinase and protein kinase C.
AB - Occurrence of two isozymic forms of fructose 6-P,2-kinase: fructose 2,6-bisphosphatase in bovine heart was investigated by transcribing mRNAs and amplifying the cDNAs with polymerase chain reactions. Analysis of the PCR products revealed 1.7 Kb and 1.5 Kb DNAs, and the determination of their nucleotide sequences showed that these DNAs are identical except for the lack of 180 base pairs near the 3′ of the bovine heart enzyme DNA previously reported (6). This missing nucleotide sequence encodes Asn451-Gln510 and contains the phosphorylation sites for cAMP dependent protein kinase and protein kinase C.
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U2 - 10.1016/S0006-291X(05)81088-5
DO - 10.1016/S0006-291X(05)81088-5
M3 - Article
C2 - 1659398
AN - SCOPUS:0025824713
SN - 0006-291X
VL - 180
SP - 470
EP - 474
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -