Characterization of two isozymic forms of heart fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase

Junichiro Sakata, Kosaku Uyeda

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Occurrence of two isozymic forms of fructose 6-P,2-kinase: fructose 2,6-bisphosphatase in bovine heart was investigated by transcribing mRNAs and amplifying the cDNAs with polymerase chain reactions. Analysis of the PCR products revealed 1.7 Kb and 1.5 Kb DNAs, and the determination of their nucleotide sequences showed that these DNAs are identical except for the lack of 180 base pairs near the 3′ of the bovine heart enzyme DNA previously reported (6). This missing nucleotide sequence encodes Asn451-Gln510 and contains the phosphorylation sites for cAMP dependent protein kinase and protein kinase C.

Original languageEnglish (US)
Pages (from-to)470-474
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number2
DOIs
StatePublished - Oct 31 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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