CHD4/NuRD maintains demethylation state of rDNA promoters through inhibiting the expression of the rDNA methyltransferase recruiter TIP5

Te Ling, Wenbing Xie, Min Luo, Meili Shen, Qiaoyun Zhu, Le Zong, Tingting Zhou, Jun Gu, Zhigang Lu, Feixiong Zhang, Wei Tao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Despite the well-established fact that NuRD (nucleosome remodeling and histone deacetylase) is incapable of actively demethylating DNA, the complex is surprisingly showed to be required for the establishment of unmethylated state at promoters of ribosomal genes. But the molecular mechanism underlying how NuRD mediates unmethylation at rDNA promoters remains obscure. Here we show that NuRD directly binds to the promoter of rDNA transcription silencer TIP5 (TTF-I interacting protein 5), one of the components of nucleolar remodeling complex NoRC that silences rRNA genes by recruiting DNA methyltransferase to rDNA promoters and increasing DNA methylation. NuRD negatively regulates TIP5 expression, thereby inhibiting rDNA methylation and maintaining demethylation state of rDNA promoters. The deficiency of NuRD components in reprogrammed cells activates TIP5 expression, resulting in the increased fraction of heterochromatic rRNA genes and transcriptional silencing. Thus, NuRD is able to control methylation status of rDNA promoters through crosstalking with NoRC complex.

Original languageEnglish (US)
Pages (from-to)101-107
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume437
Issue number1
DOIs
StatePublished - Jul 19 2013

Fingerprint

Histone Deacetylases
Nucleosomes
Methyltransferases
Ribosomal DNA
Proteins
Methylation
Genes
rRNA Genes
DNA
Gene Silencing
DNA Methylation
Transcription

Keywords

  • DNA methylation
  • NoRC
  • Reprogramming
  • rRNA genes
  • TIP5

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

CHD4/NuRD maintains demethylation state of rDNA promoters through inhibiting the expression of the rDNA methyltransferase recruiter TIP5. / Ling, Te; Xie, Wenbing; Luo, Min; Shen, Meili; Zhu, Qiaoyun; Zong, Le; Zhou, Tingting; Gu, Jun; Lu, Zhigang; Zhang, Feixiong; Tao, Wei.

In: Biochemical and Biophysical Research Communications, Vol. 437, No. 1, 19.07.2013, p. 101-107.

Research output: Contribution to journalArticle

Ling, Te ; Xie, Wenbing ; Luo, Min ; Shen, Meili ; Zhu, Qiaoyun ; Zong, Le ; Zhou, Tingting ; Gu, Jun ; Lu, Zhigang ; Zhang, Feixiong ; Tao, Wei. / CHD4/NuRD maintains demethylation state of rDNA promoters through inhibiting the expression of the rDNA methyltransferase recruiter TIP5. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 437, No. 1. pp. 101-107.
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AU - Zhu, Qiaoyun

AU - Zong, Le

AU - Zhou, Tingting

AU - Gu, Jun

AU - Lu, Zhigang

AU - Zhang, Feixiong

AU - Tao, Wei

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