Chemical activity of cholesterol in membranes

Arun Radhakrishnan, Harden M. McConnell

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

Measurements are reported for the rate constants for the release of cholesterol (and dihydrocholesterol) to β-cyclodextrin from mixtures with phospholipids in homogeneous monolayers at constant pressure at the air-water interface. In each mixture, it is found that the release rate shows a sharp decrease as the cholesterol concentration in the monolayer decreases through a composition corresponding to the stoichiometry of a cholesterol- phospholipid complex. The stoichiometry of the complex was established previously by the position of a sharp cusp in the thermodynamic phase diagram of each mixture and also by a minimum in average molecular area versus composition measurements. A theoretical model used earlier to account for the phase diagrams predicts the chemical potential and chemical activity of cholesterol in these mixtures. The calculated chemical activity also shows a sharp change at the complex stoichiometry in homogeneous monolayers. The similarities in change of observed release rate and calculated chemical activity are expected from reaction rate theory where the release rate is proportional to the cholesterol chemical activity. The chemical activity of cholesterol as determined by complex formation between some phospholipids and cholesterol in the plasma membrane of cells may serve a regulatory function with respect to intracellular cholesterol transport and biosynthesis.

Original languageEnglish (US)
Pages (from-to)8119-8124
Number of pages6
JournalBiochemistry
Volume39
Issue number28
DOIs
StatePublished - Jul 18 2000

ASJC Scopus subject areas

  • Biochemistry

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