A method for the solid phase chemical synthesis of AMPylated Ser/Thr containing peptides is described. Peptides were phosphonylated using the H-phosphonate method, then the adenosine moiety was introduced using 2′,3′-isopropylidene adenosine and the condensing reagent PyBOP. Oxidation with iodine yielded the desired AMPylated product in good yield following acidolytic cleavage from the resin support.
- 2′,3′-Isopropylidene adenosine
- Benzotriazole-1-yl oxy-tris-pyrrolidino-phosphonium hexafluorophosphate (PyBOP)
- Bis(diethylamino) chlorophosphine
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry