TY - JOUR
T1 - Chemically synthesised human immunodeficiency virus P7 nucleocapsid protein can self-assemble into particle sand binds to a specific site on the tRNA(LyS,3) primer
AU - Al-Ghusein, Hasan
AU - Ball, Haydn
AU - Igloi, Gabor L.
AU - Gbewonyo, Armstrong
AU - Coates, Anthony R M
AU - Mascagni, Paolo
AU - Roberts, Michael M.
N1 - Funding Information:
We thank Dr. Milan V. Nermut and Dr. Sean Heaphy for helpful discussion on the p7 aggregation and p7-tRNALys,3 binding. The assistance of the laboratory staff at Italfarmaco is gratefully acknowledged. We are also grateful to Miss E. Hutchinson for the zinc analyses on HIV p7. This work was supported by Grant G9304370 from the Medical Research Council.
PY - 1996/7/5
Y1 - 1996/7/5
N2 - The zinc-bound form of the human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein, p7, aggregates into particles visible by electron microscopy. The HIV primer tRNA(Lys,3) forms similar high molecular weight complexes with p7 that are also detected by gel mobility shift assays. RNA oligonucleotides of the three stem-loop structures in tRNA(Lys,3) were assayed for the competitive inhibition of p7-tRNA(Lys,3) binding by the intensities of free tRNA(Lys,3) bands on native gels. This reveals that the p7 binds specifically to the central domain of tRNA(Lys,3) where the D and TΨC loops come together, but not the anticodon stem-loop.
AB - The zinc-bound form of the human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein, p7, aggregates into particles visible by electron microscopy. The HIV primer tRNA(Lys,3) forms similar high molecular weight complexes with p7 that are also detected by gel mobility shift assays. RNA oligonucleotides of the three stem-loop structures in tRNA(Lys,3) were assayed for the competitive inhibition of p7-tRNA(Lys,3) binding by the intensities of free tRNA(Lys,3) bands on native gels. This reveals that the p7 binds specifically to the central domain of tRNA(Lys,3) where the D and TΨC loops come together, but not the anticodon stem-loop.
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U2 - 10.1006/bbrc.1996.1006
DO - 10.1006/bbrc.1996.1006
M3 - Article
C2 - 8694811
AN - SCOPUS:0030570446
SN - 0006-291X
VL - 224
SP - 191
EP - 198
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -