Chemiluminescence-based detection of gastrointestinal malignancies

Jason Y. Park, Thomas J. Kirn, David Artis, Scott A. Waldman, Larry J. Kricka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Chemiluminescence is an established method for the in vitro serum monitoring of human tumors. Chemiluminescence may have additional utility for the in vivo detection of tumors. During carcinogenesis, tumors change their phenotype and the proteins they express. Specifically, in carcinogenesis of the esophagus and stomach, enzymes that are normally only expressed in the small intestine brush border become ectopically expressed in precancerous and cancerous lesions. Intestinal alkaline phosphatase and lactase are among the small intestine brush border enzymes that are ectopically expressed. We have found that specific chemiluminescent substrates for alkaline phosphatase and lactase may be used for the in situ detection of intestinal alkaline phosphatase and lactase in unprocessed tissue. In this study, we demonstrate that chemiluminescent 1,2-dioxetane substrates may be used for the detection of precancerous and cancerous lesions from the esophagus and stomach.

Original languageEnglish (US)
Pages (from-to)463-465
Number of pages3
JournalLuminescence
Volume25
Issue number6
DOIs
StatePublished - Nov 2010

Fingerprint

Lactase
Chemiluminescence
Luminescence
Alkaline Phosphatase
Tumors
Brushes
Microvilli
Esophagus
Small Intestine
Stomach
Carcinogenesis
Neoplasms
Substrates
Enzymes
Tissue
Phenotype
Monitoring
Serum
Proteins

Keywords

  • cancer
  • chemiluminescence
  • dioxetane
  • gastrointestinal
  • in vivo

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)

Cite this

Park, J. Y., Kirn, T. J., Artis, D., Waldman, S. A., & Kricka, L. J. (2010). Chemiluminescence-based detection of gastrointestinal malignancies. Luminescence, 25(6), 463-465. https://doi.org/10.1002/bio.1173

Chemiluminescence-based detection of gastrointestinal malignancies. / Park, Jason Y.; Kirn, Thomas J.; Artis, David; Waldman, Scott A.; Kricka, Larry J.

In: Luminescence, Vol. 25, No. 6, 11.2010, p. 463-465.

Research output: Contribution to journalArticle

Park, JY, Kirn, TJ, Artis, D, Waldman, SA & Kricka, LJ 2010, 'Chemiluminescence-based detection of gastrointestinal malignancies', Luminescence, vol. 25, no. 6, pp. 463-465. https://doi.org/10.1002/bio.1173
Park, Jason Y. ; Kirn, Thomas J. ; Artis, David ; Waldman, Scott A. ; Kricka, Larry J. / Chemiluminescence-based detection of gastrointestinal malignancies. In: Luminescence. 2010 ; Vol. 25, No. 6. pp. 463-465.
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