Chemoattractant agents and nerve growth factor stimulate human spermatozoal reactive oxygen species generation

D. L. Weese, M. L. Peaster, R. D. Hernandez, G. E. Leach, P. M. Lad, P. E. Zimmern

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: To investigate the ability of N-formyl-methionyl-leucyl- phenylalanine (f-MLP), complement 5a (C5a), and nerve growth factor (NGF) to stimulate human spermatozoal reactive oxygen species generation in fertile and infertile patients. Design: Prospective, controlled study measuring human spermatozoal reactive oxygen species generation after addition of f-MLP, C5a, or NGF. Setting: A large health maintenance organization. Patients, Participants: The fertile group consisted of 14 men with established fertility and normal bulk semen parameters. The infertile group was comprised of 8 men who were infertile after >18 months of unprotected sexual intercourse. Interventions: The sperm samples were subjected to four test conditions: f-MLP stimulation, C5a stimulation, NGF stimulation, and no stimulation (control). Main Outcome Measure: Reactive oxygen generation was measured over a 15-minute period using the method of chemiluminescence. Results: In both the fertile and infertile groups, reactive oxygen species generation was significantly enhanced by f-MLP, C5a, and NGF compared with controls. No significant difference in f-MLP- and C5a-stimulated reactive oxygen production was demonstrated between the infertile and fertile groups; however, there was a significant difference in reactive oxygen generation between infertile and fertile subjects when stimulated with NGF. Conclusions: The current study represents the first report of f-MLP-, C5a-, and NGF- stimulated reactive oxygen species generation by human spermatozoa. Nerve growth factor enhanced reactive oxygen species production to a greater extent in infertile subjects compared with fertile subjects. This points to a possible NGF-mediated biochemical defect in the sperm of infertile patients.

Original languageEnglish (US)
Pages (from-to)869-875
Number of pages7
JournalFertility and Sterility
Volume59
Issue number4
StatePublished - 1993

Fingerprint

Complement C5a
Chemotactic Factors
Nerve Growth Factor
methionyl-leucyl-phenylalanine
Reactive Oxygen Species
Spermatozoa
Oxygen
N-Formylmethionine Leucyl-Phenylalanine
Aptitude
Health Maintenance Organizations
Coitus
Luminescence
Semen
Fertility
Outcome Assessment (Health Care)
Prospective Studies

Keywords

  • C5a
  • chemoattractants
  • complement 5a
  • f-MLP
  • Nerve growth factor
  • oxygen radicals
  • reactive oxygen generation
  • spermatozoa
  • superoxide generation

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Weese, D. L., Peaster, M. L., Hernandez, R. D., Leach, G. E., Lad, P. M., & Zimmern, P. E. (1993). Chemoattractant agents and nerve growth factor stimulate human spermatozoal reactive oxygen species generation. Fertility and Sterility, 59(4), 869-875.

Chemoattractant agents and nerve growth factor stimulate human spermatozoal reactive oxygen species generation. / Weese, D. L.; Peaster, M. L.; Hernandez, R. D.; Leach, G. E.; Lad, P. M.; Zimmern, P. E.

In: Fertility and Sterility, Vol. 59, No. 4, 1993, p. 869-875.

Research output: Contribution to journalArticle

Weese, DL, Peaster, ML, Hernandez, RD, Leach, GE, Lad, PM & Zimmern, PE 1993, 'Chemoattractant agents and nerve growth factor stimulate human spermatozoal reactive oxygen species generation', Fertility and Sterility, vol. 59, no. 4, pp. 869-875.
Weese, D. L. ; Peaster, M. L. ; Hernandez, R. D. ; Leach, G. E. ; Lad, P. M. ; Zimmern, P. E. / Chemoattractant agents and nerve growth factor stimulate human spermatozoal reactive oxygen species generation. In: Fertility and Sterility. 1993 ; Vol. 59, No. 4. pp. 869-875.
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AU - Weese, D. L.

AU - Peaster, M. L.

AU - Hernandez, R. D.

AU - Leach, G. E.

AU - Lad, P. M.

AU - Zimmern, P. E.

PY - 1993

Y1 - 1993

N2 - Objective: To investigate the ability of N-formyl-methionyl-leucyl- phenylalanine (f-MLP), complement 5a (C5a), and nerve growth factor (NGF) to stimulate human spermatozoal reactive oxygen species generation in fertile and infertile patients. Design: Prospective, controlled study measuring human spermatozoal reactive oxygen species generation after addition of f-MLP, C5a, or NGF. Setting: A large health maintenance organization. Patients, Participants: The fertile group consisted of 14 men with established fertility and normal bulk semen parameters. The infertile group was comprised of 8 men who were infertile after >18 months of unprotected sexual intercourse. Interventions: The sperm samples were subjected to four test conditions: f-MLP stimulation, C5a stimulation, NGF stimulation, and no stimulation (control). Main Outcome Measure: Reactive oxygen generation was measured over a 15-minute period using the method of chemiluminescence. Results: In both the fertile and infertile groups, reactive oxygen species generation was significantly enhanced by f-MLP, C5a, and NGF compared with controls. No significant difference in f-MLP- and C5a-stimulated reactive oxygen production was demonstrated between the infertile and fertile groups; however, there was a significant difference in reactive oxygen generation between infertile and fertile subjects when stimulated with NGF. Conclusions: The current study represents the first report of f-MLP-, C5a-, and NGF- stimulated reactive oxygen species generation by human spermatozoa. Nerve growth factor enhanced reactive oxygen species production to a greater extent in infertile subjects compared with fertile subjects. This points to a possible NGF-mediated biochemical defect in the sperm of infertile patients.

AB - Objective: To investigate the ability of N-formyl-methionyl-leucyl- phenylalanine (f-MLP), complement 5a (C5a), and nerve growth factor (NGF) to stimulate human spermatozoal reactive oxygen species generation in fertile and infertile patients. Design: Prospective, controlled study measuring human spermatozoal reactive oxygen species generation after addition of f-MLP, C5a, or NGF. Setting: A large health maintenance organization. Patients, Participants: The fertile group consisted of 14 men with established fertility and normal bulk semen parameters. The infertile group was comprised of 8 men who were infertile after >18 months of unprotected sexual intercourse. Interventions: The sperm samples were subjected to four test conditions: f-MLP stimulation, C5a stimulation, NGF stimulation, and no stimulation (control). Main Outcome Measure: Reactive oxygen generation was measured over a 15-minute period using the method of chemiluminescence. Results: In both the fertile and infertile groups, reactive oxygen species generation was significantly enhanced by f-MLP, C5a, and NGF compared with controls. No significant difference in f-MLP- and C5a-stimulated reactive oxygen production was demonstrated between the infertile and fertile groups; however, there was a significant difference in reactive oxygen generation between infertile and fertile subjects when stimulated with NGF. Conclusions: The current study represents the first report of f-MLP-, C5a-, and NGF- stimulated reactive oxygen species generation by human spermatozoa. Nerve growth factor enhanced reactive oxygen species production to a greater extent in infertile subjects compared with fertile subjects. This points to a possible NGF-mediated biochemical defect in the sperm of infertile patients.

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KW - chemoattractants

KW - complement 5a

KW - f-MLP

KW - Nerve growth factor

KW - oxygen radicals

KW - reactive oxygen generation

KW - spermatozoa

KW - superoxide generation

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M3 - Article

VL - 59

SP - 869

EP - 875

JO - Fertility and Sterility

JF - Fertility and Sterility

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