Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis

Ya Xia Tan, Wen Ling Han, Ying Yu Chen, Neng Tai Ouyang, Yan Tang, Feng Li, Pei Guo Ding, Xiao Ian Ren, Guang Qiao Zeng, Jing Ding, Tong Zhu, Da Long Ma, Nan Shan Zhong

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background. Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine. The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines. Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells. We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung. Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung. Methods. CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation. Lung tissues were obtained at the end of week 1,2,3 and 4 respectively after injection. The pathological changes in the lungs were observed by light microscope. Results. A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice. These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung. Conclusions. The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome. These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electrotransfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals.

Original languageEnglish (US)
Pages (from-to)1123-1129
Number of pages7
JournalChinese Medical Journal
Volume117
Issue number8
StatePublished - Aug 2004

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Airway Remodeling
Pulmonary Fibrosis
Chemokines
Cytokines
Lung
Skeletal Muscle
Leukocytes
Plasmids
Genes
Severe Acute Respiratory Syndrome
CC Chemokines
Electroporation
Intramuscular Injections
Interstitial Lung Diseases
Adult Respiratory Distress Syndrome
Muscle Cells
Smooth Muscle Myocytes
Fibrosis
Collagen
Asthma

Keywords

  • Airway remodeling
  • Asthma
  • Chemokine-like factor 1
  • Electroporation
  • Pathology
  • Severe acute respiratory syndrome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tan, Y. X., Han, W. L., Chen, Y. Y., Ouyang, N. T., Tang, Y., Li, F., ... Zhong, N. S. (2004). Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis. Chinese Medical Journal, 117(8), 1123-1129.

Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis. / Tan, Ya Xia; Han, Wen Ling; Chen, Ying Yu; Ouyang, Neng Tai; Tang, Yan; Li, Feng; Ding, Pei Guo; Ren, Xiao Ian; Zeng, Guang Qiao; Ding, Jing; Zhu, Tong; Ma, Da Long; Zhong, Nan Shan.

In: Chinese Medical Journal, Vol. 117, No. 8, 08.2004, p. 1123-1129.

Research output: Contribution to journalArticle

Tan, YX, Han, WL, Chen, YY, Ouyang, NT, Tang, Y, Li, F, Ding, PG, Ren, XI, Zeng, GQ, Ding, J, Zhu, T, Ma, DL & Zhong, NS 2004, 'Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis', Chinese Medical Journal, vol. 117, no. 8, pp. 1123-1129.
Tan YX, Han WL, Chen YY, Ouyang NT, Tang Y, Li F et al. Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis. Chinese Medical Journal. 2004 Aug;117(8):1123-1129.
Tan, Ya Xia ; Han, Wen Ling ; Chen, Ying Yu ; Ouyang, Neng Tai ; Tang, Yan ; Li, Feng ; Ding, Pei Guo ; Ren, Xiao Ian ; Zeng, Guang Qiao ; Ding, Jing ; Zhu, Tong ; Ma, Da Long ; Zhong, Nan Shan. / Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis. In: Chinese Medical Journal. 2004 ; Vol. 117, No. 8. pp. 1123-1129.
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abstract = "Background. Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine. The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines. Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells. We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung. Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung. Methods. CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation. Lung tissues were obtained at the end of week 1,2,3 and 4 respectively after injection. The pathological changes in the lungs were observed by light microscope. Results. A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice. These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung. Conclusions. The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome. These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electrotransfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals.",
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AU - Tan, Ya Xia

AU - Han, Wen Ling

AU - Chen, Ying Yu

AU - Ouyang, Neng Tai

AU - Tang, Yan

AU - Li, Feng

AU - Ding, Pei Guo

AU - Ren, Xiao Ian

AU - Zeng, Guang Qiao

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AU - Zhu, Tong

AU - Ma, Da Long

AU - Zhong, Nan Shan

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AB - Background. Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine. The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines. Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells. We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung. Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung. Methods. CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation. Lung tissues were obtained at the end of week 1,2,3 and 4 respectively after injection. The pathological changes in the lungs were observed by light microscope. Results. A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice. These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung. Conclusions. The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome. These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electrotransfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals.

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