Chemokine receptor CXCR4 signaling modulates the growth factor-induced cell cycle of self-renewing and multipotent neural progenitor cells

Meizhang Li, Cathleen J. Chang, Justin D. Lathia, Li Wang, Holly L. Pacenta, Anne Cotleur, Richard M. Ransohoff

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

CXC chemokine receptor CXCR4 is expressed in vitro in both human and rodent adult neural progenitor cells (NPCs). It has been suggested that the CXCL12-CXCR4 axis potentially enhances the proliferation of NPCs. However, whether CXCR4 is expressed in the neural stem cells (NSCs), a subset of self-renewing and multipotent NPCs, and whether CXCR4 signaling is directly required for their proliferation are not clear. In this study, we report that CXCR4 is expressed in a subpopulation of NPCs in the early embryonic ventricular zone. In studies of a CXCR4eGFP bacterial artificial chromosomal (BAC) transgenic mouse line, we further isolated NPCs from E12.5 transgenic telencephalon and GFP+ cells demonstrated self-renewal and multipotency in neurosphere assays in vitro. Consistent with these observations, we enriched GFP+/CXCR4+ cells by fluorescence activated cell sorting (FACS) with either CXCR4 antibody 12G5 or GFP. Furthermore, we observed that CXCL12 alone did not activate the self-renewal of NPCs or increase the proliferation of NPCs that are induced by bFGF/EGF. However, we found that blocking CXCR4 receptor with antagonist AMD3100 impaired the bFGF/EGF-induced expansion of GFP+ NPCs through modulating their cell cycling. In addition, AMD3100 treatment of pregnant mice reduced the generation of neurospheres from E12.5 embryos. Our data suggest that CXCR4 is a potential cell surface marker for early embryonic NSCs and modulates growth-factor signaling.

Original languageEnglish (US)
Pages (from-to)108-118
Number of pages11
JournalGLIA
Volume59
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Keywords

  • CXCL12
  • CXCR4
  • Cell cycling
  • Neural progenitor cells
  • Proliferation

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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    Li, M., Chang, C. J., Lathia, J. D., Wang, L., Pacenta, H. L., Cotleur, A., & Ransohoff, R. M. (2011). Chemokine receptor CXCR4 signaling modulates the growth factor-induced cell cycle of self-renewing and multipotent neural progenitor cells. GLIA, 59(1), 108-118. https://doi.org/10.1002/glia.21080