Chemosensitivity patterns and expression of human multidrug resistance-associated MDR1 gene by human gastric and colorectal carcinoma cell lines

Jae Gahb Park, Barnett S. Kramer, Shinn Liang Lai, Lori J. Goldstein, Adi F. Gazdar

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We compared the in vitro sensitivity patterns to cytotoxic drugs and expression of the multidrug resistance-associated MDR1 gene (also known as PGY1 gene) in four gastric carcinoma cell lines with those obtained in a panel of 11 colorectal carcinoma cell lines. In addition, we tested the effects of leucovorin on enhancement of fluorinated pyrimidine-induced cytotoxicity. We used a semiautomated tetrazolium dye assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (tetrazolyl blue)] (MTT) to compare the drug sensitivity of the gastric carcinoma cell lines with that of the colorectal carcinoma cell lines. The gastric carcinoma cell lines were more sensitive to some drugs, including doxorubicin and cisplatin, but not to the fluorinated pyrimidines. Addition of leucovorin at a clinically achievable concentration enhanced the cytotoxic effects of both fluorouracil and floxuridine in colorectal carcinoma cell lines, but it enhanced the effects of only floxuridine in gastric carcinoma cell lines. With the use of a slot blot assay, relatively low levels of MDR1 RNA were present in all four gastric carcinoma cell lines, while intermediate or high levels were present in most of the colorectal carcinoma cell lines. In general, our findings reflect clinical experience and may help in the design of clinical trials.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalJournal of the National Cancer Institute
Volume82
Issue number3
StatePublished - Feb 7 1990

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Multiple Drug Resistance
Colorectal Neoplasms
Stomach
Genes
Cells
Gene
Cell Line
Line
Cell
Carcinoma
Floxuridine
Drugs
Leucovorin
Assays
Cisplatin
Gastrointestinal Agents
Resistance
Human
Cell line
Multi-target Tracking

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging

Cite this

Chemosensitivity patterns and expression of human multidrug resistance-associated MDR1 gene by human gastric and colorectal carcinoma cell lines. / Park, Jae Gahb; Kramer, Barnett S.; Lai, Shinn Liang; Goldstein, Lori J.; Gazdar, Adi F.

In: Journal of the National Cancer Institute, Vol. 82, No. 3, 07.02.1990, p. 193-198.

Research output: Contribution to journalArticle

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