Chick chorioallantoic membrane as an in vivo model to study vasoreactivity: Characterization of development-dependent hyperemia induced by epoxyeicosatrienoic acids (EETs)

Laurel K. Dunn, Stephanie K. Gruenloh, Bruce E. Dunn, D. Sudarshan Reddy, J R Falck, Elizabeth R. Jacobs, Meetha Medhora

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Shell-less culture of chick chorioallantoic membrane (CAM) of developing chicken embryos is a useful model to evaluate the effects of vascular agents. We assessed the response of CAM vessels to epoxyeicosatrienoic acids (EETs), derivatives of the essential fatty acid arachidonic acid, that have a number of important biological functions, including dilation of microvessels in the coronary, cerebral, renal, and mesenteric circulations. Three of four regioisomers of EETs, 14,15-, 11,12-, and 8,9-EET, induced a characteristic dose-dependent acute hyperemia within 4 min after application on 10-day-old CAMs. This response was marked in early stages of development (between days 8 and 10), but the frequency and intensity of the response were reduced after 11 days of development. Histological examination demonstrated that the hyperemia was not due to extravasation of erythrocytes. However, many capillaries were distended and contained densely packed erythrocytes as compared to uniformly arranged vessels and erythrocytes in untreated CAMs. Transmission electron microscopy showed the basal laminae surrounding capillaries remained intact, similar to those in vehicle-treated or untreated CAM tissue. The hyperemia was specific to EETs since we did not observe it to be induced by other vasodilators such as nitric oxide or prostacyclin. In conclusion, we report a novel vascular response to EETs using the CAM as an in vivo model. These lipids specifically distend a subset of capillaries in a dose- and development-dependent manner.

Original languageEnglish (US)
Pages (from-to)771-780
Number of pages10
JournalAnatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume285
Issue number2
DOIs
StatePublished - Aug 2005

Fingerprint

Chorioallantoic Membrane
chorioallantoic membrane
Hyperemia
chicks
membrane
Acids
erythrocytes
Erythrocytes
acids
acid
blood vessels
Blood Vessels
Cerebrovascular Circulation
vessel
Splanchnic Circulation
prostacyclin
Coronary Circulation
vasodilator agents
Essential Fatty Acids
Renal Circulation

Keywords

  • Chorioallantoic membrane
  • Embryonic
  • Endothelial cell distension
  • Vasoreactivity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Chick chorioallantoic membrane as an in vivo model to study vasoreactivity : Characterization of development-dependent hyperemia induced by epoxyeicosatrienoic acids (EETs). / Dunn, Laurel K.; Gruenloh, Stephanie K.; Dunn, Bruce E.; Reddy, D. Sudarshan; Falck, J R; Jacobs, Elizabeth R.; Medhora, Meetha.

In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, Vol. 285, No. 2, 08.2005, p. 771-780.

Research output: Contribution to journalArticle

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