Chicken liver phosphofructokinase. I. Crystallization and physicochemical properties

N. Kono, K. Uyeda, R. M. Oliver

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Chicken liver and breast muscle phosphofructokinases were purified to homogeneity; the liver enzyme was crystallized. The liver phosphofructokinase is reversibly inactivated at low temperatures. This property appears to be unique to phosphofructokinases from avian livers; the enzyme from other tissues of chicken and from the livers of several other animals are stable in the cold. Sucrose density gradient centrifugation, gel filtration, and ultracentrifugal analysis reveal polymeric forms of the chicken liver enzyme, with the distribution depending upon the conditions and protein concentration. The molecular weight of the monomeric form of the liver enzyme is approximately 400,000. However, the enzyme in NaHCO3 at pH 10.5 has a molecular weight of about 210,000. In 7 M guanidine hydrochloride the enzyme dissociates into subunits of 60,000 ± 5,000 daltons. Peptide mapping and gel electrophoresis in sodium dodecyl sulfate suggest that these subunits are identical. Negative stain electron microscopy indicates that stable dimers and tetramers are formed by self association of these subunits. Under appropriate conditions, the higher polymeric forms result from aggregation of the tetramers into chains or sheets.

Original languageEnglish (US)
Pages (from-to)8592-8602
Number of pages11
JournalJournal of Biological Chemistry
Volume248
Issue number24
StatePublished - 1973

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Phosphofructokinases
Crystallization
Liver
Chickens
Enzymes
Molecular Weight
Gels
Molecular weight
Peptide Mapping
Density Gradient Centrifugation
Centrifugation
Guanidine
Electrophoresis
Sodium Dodecyl Sulfate
Dimers
Electron microscopy
Gel Chromatography
Sucrose
Muscle
Electron Microscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chicken liver phosphofructokinase. I. Crystallization and physicochemical properties. / Kono, N.; Uyeda, K.; Oliver, R. M.

In: Journal of Biological Chemistry, Vol. 248, No. 24, 1973, p. 8592-8602.

Research output: Contribution to journalArticle

Kono, N. ; Uyeda, K. ; Oliver, R. M. / Chicken liver phosphofructokinase. I. Crystallization and physicochemical properties. In: Journal of Biological Chemistry. 1973 ; Vol. 248, No. 24. pp. 8592-8602.
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