Chikungunya virus-induced autophagy delays caspase-dependent cell death

Pierre Emmanuel Joubert, Scott W. Werneke, Claire de la Calle, Florence Guivel-Benhassine, Alessandra Giodini, Lucie Peduto, Beth Levine, Olivier Schwartz, Deborah J. Lenschow, Matthew L. Albert

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Autophagy is an important survival pathway and can participate in the host response to infection. Studying Chikungunya virus (CHIKV), the causative agent of a major epidemic in India, Southeast Asia, and southern Europe, we reveal a novel mechanism by which autophagy limits cell death and mortality after infection. We use biochemical studies and single cell multispectral assays to demonstrate that direct infection triggers both apoptosis and autophagy. CHIKVinduced autophagy is mediated by the independent induction of endoplasmic reticulum and oxidative stress pathways. These cellular responses delay apoptotic cell death by inducing the IRE1α-XBP-1 pathway in conjunction with ROS-mediated mTOR inhibition. Silencing of autophagy genes resulted in enhanced intrinsic and extrinsic apoptosis, favoring viral propagation in cultured cells. Providing in vivo evidence for the relevance of our findings, Atg16LHM mice, which display reduced levels of autophagy, exhibited increased lethality and showed a higher sensitivity to CHIKV-induced apoptosis. Based on kinetic studies and the observation that features of apoptosis and autophagy were mutually exclusive, we conclude that autophagy inhibits caspase-dependent cell death but is ultimately overwhelmed by viral replication. Our study suggests that inducers of autophagy may limit the pathogenesis of acute Chikungunya disease.

Original languageEnglish (US)
Pages (from-to)1029-1047
Number of pages19
JournalJournal of Experimental Medicine
Volume209
Issue number5
DOIs
StatePublished - May 7 2012

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Chikungunya virus
Autophagy
Caspases
Cell Death
Apoptosis
Infection
Southeastern Asia
Endoplasmic Reticulum Stress
Gene Silencing
Acute Disease
India
Cultured Cells
Oxidative Stress

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Joubert, P. E., Werneke, S. W., de la Calle, C., Guivel-Benhassine, F., Giodini, A., Peduto, L., ... Albert, M. L. (2012). Chikungunya virus-induced autophagy delays caspase-dependent cell death. Journal of Experimental Medicine, 209(5), 1029-1047. https://doi.org/10.1084/jem.20110996

Chikungunya virus-induced autophagy delays caspase-dependent cell death. / Joubert, Pierre Emmanuel; Werneke, Scott W.; de la Calle, Claire; Guivel-Benhassine, Florence; Giodini, Alessandra; Peduto, Lucie; Levine, Beth; Schwartz, Olivier; Lenschow, Deborah J.; Albert, Matthew L.

In: Journal of Experimental Medicine, Vol. 209, No. 5, 07.05.2012, p. 1029-1047.

Research output: Contribution to journalArticle

Joubert, PE, Werneke, SW, de la Calle, C, Guivel-Benhassine, F, Giodini, A, Peduto, L, Levine, B, Schwartz, O, Lenschow, DJ & Albert, ML 2012, 'Chikungunya virus-induced autophagy delays caspase-dependent cell death', Journal of Experimental Medicine, vol. 209, no. 5, pp. 1029-1047. https://doi.org/10.1084/jem.20110996
Joubert PE, Werneke SW, de la Calle C, Guivel-Benhassine F, Giodini A, Peduto L et al. Chikungunya virus-induced autophagy delays caspase-dependent cell death. Journal of Experimental Medicine. 2012 May 7;209(5):1029-1047. https://doi.org/10.1084/jem.20110996
Joubert, Pierre Emmanuel ; Werneke, Scott W. ; de la Calle, Claire ; Guivel-Benhassine, Florence ; Giodini, Alessandra ; Peduto, Lucie ; Levine, Beth ; Schwartz, Olivier ; Lenschow, Deborah J. ; Albert, Matthew L. / Chikungunya virus-induced autophagy delays caspase-dependent cell death. In: Journal of Experimental Medicine. 2012 ; Vol. 209, No. 5. pp. 1029-1047.
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