Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation

Sherrie L. Perkins; Diane Pickering; Eric J. Lowe; David Zwick; Minnie Abromowitch; Ginny Davenport; Mitchell S. Cairo; Warren G. Sanger

doi:10.1111/j.1365-2141.2005.05808.x

Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation

Sherrie L. Perkins, Diane Pickering, Eric J. Lowe, David Zwick, Minnie Abromowitch, Ginny Davenport, Mitchell S. Cairo, Warren G. Sanger

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Abstract

Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.

Original language	English (US)
Pages (from-to)	624-627
Number of pages	4
Journal	British Journal of Haematology
Volume	131
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2141.2005.05808.x
State	Published - Dec 2005

Keywords

ALK
Anaplastic large cell lymphoma
Fluorescent in situ hybridisation
Immunochemistry
Paediatric non-Hodgkin lymphoma

ASJC Scopus subject areas

Hematology

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Perkins, S. L., Pickering, D., Lowe, E. J., Zwick, D., Abromowitch, M., Davenport, G., Cairo, M. S., & Sanger, W. G. (2005). Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation. British Journal of Haematology, 131(5), 624-627. https://doi.org/10.1111/j.1365-2141.2005.05808.x

Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation. / Perkins, Sherrie L.; Pickering, Diane; Lowe, Eric J. et al.
In: British Journal of Haematology, Vol. 131, No. 5, 12.2005, p. 624-627.

Research output: Contribution to journal › Article › peer-review

Perkins, SL, Pickering, D, Lowe, EJ, Zwick, D, Abromowitch, M, Davenport, G, Cairo, MS & Sanger, WG 2005, 'Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation', British Journal of Haematology, vol. 131, no. 5, pp. 624-627. https://doi.org/10.1111/j.1365-2141.2005.05808.x

Perkins SL, Pickering D, Lowe EJ, Zwick D, Abromowitch M, Davenport G et al. Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation. British Journal of Haematology. 2005 Dec;131(5):624-627. doi: 10.1111/j.1365-2141.2005.05808.x

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title = "Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation",

abstract = "Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.",

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author = "Perkins, {Sherrie L.} and Diane Pickering and Lowe, {Eric J.} and David Zwick and Minnie Abromowitch and Ginny Davenport and Cairo, {Mitchell S.} and Sanger, {Warren G.}",

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AU - Lowe, Eric J.

AU - Zwick, David

AU - Abromowitch, Minnie

AU - Davenport, Ginny

AU - Cairo, Mitchell S.

AU - Sanger, Warren G.

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AB - Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.

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Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation

Abstract

Keywords

ASJC Scopus subject areas

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