Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation

Sherrie L. Perkins, Diane Pickering, Eric J. Lowe, David Zwick, Minnie Abromowitch, Ginny Davenport, Mitchell S. Cairo, Warren G. Sanger

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.

Original languageEnglish (US)
Pages (from-to)624-627
Number of pages4
JournalBritish Journal of Haematology
Volume131
Issue number5
DOIs
StatePublished - Dec 1 2005

Fingerprint

Anaplastic Large-Cell Lymphoma
Gene Rearrangement
Fluorescence In Situ Hybridization
Staining and Labeling
Incidence
anaplastic lymphoma kinase
Non-Hodgkin's Lymphoma
Immunohistochemistry
Pediatrics

Keywords

  • ALK
  • Anaplastic large cell lymphoma
  • Fluorescent in situ hybridisation
  • Immunochemistry
  • Paediatric non-Hodgkin lymphoma

ASJC Scopus subject areas

  • Hematology

Cite this

Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation : A genetic and pathological correlation. / Perkins, Sherrie L.; Pickering, Diane; Lowe, Eric J.; Zwick, David; Abromowitch, Minnie; Davenport, Ginny; Cairo, Mitchell S.; Sanger, Warren G.

In: British Journal of Haematology, Vol. 131, No. 5, 01.12.2005, p. 624-627.

Research output: Contribution to journalArticle

Perkins, Sherrie L. ; Pickering, Diane ; Lowe, Eric J. ; Zwick, David ; Abromowitch, Minnie ; Davenport, Ginny ; Cairo, Mitchell S. ; Sanger, Warren G. / Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation : A genetic and pathological correlation. In: British Journal of Haematology. 2005 ; Vol. 131, No. 5. pp. 624-627.
@article{7dbbb63cf1694a5893282c8f675cbd97,
title = "Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: A genetic and pathological correlation",
abstract = "Anaplastic large cell lymphoma (ALCL) comprises 10-15{\%} of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83{\%}). Immunohistochemistry identified ALK over-expression in 42/43 cases (97{\%}) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100{\%} incidence of ALK translocations.",
keywords = "ALK, Anaplastic large cell lymphoma, Fluorescent in situ hybridisation, Immunochemistry, Paediatric non-Hodgkin lymphoma",
author = "Perkins, {Sherrie L.} and Diane Pickering and Lowe, {Eric J.} and David Zwick and Minnie Abromowitch and Ginny Davenport and Cairo, {Mitchell S.} and Sanger, {Warren G.}",
year = "2005",
month = "12",
day = "1",
doi = "10.1111/j.1365-2141.2005.05808.x",
language = "English (US)",
volume = "131",
pages = "624--627",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation

T2 - A genetic and pathological correlation

AU - Perkins, Sherrie L.

AU - Pickering, Diane

AU - Lowe, Eric J.

AU - Zwick, David

AU - Abromowitch, Minnie

AU - Davenport, Ginny

AU - Cairo, Mitchell S.

AU - Sanger, Warren G.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.

AB - Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.

KW - ALK

KW - Anaplastic large cell lymphoma

KW - Fluorescent in situ hybridisation

KW - Immunochemistry

KW - Paediatric non-Hodgkin lymphoma

UR - http://www.scopus.com/inward/record.url?scp=33644812125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644812125&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2141.2005.05808.x

DO - 10.1111/j.1365-2141.2005.05808.x

M3 - Article

C2 - 16351638

AN - SCOPUS:33644812125

VL - 131

SP - 624

EP - 627

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 5

ER -