Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes

John D. Minna, Thomas H. Marshall, Patricia V. Shaffer-Berman

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and one pair (PGD-PGM2) to be syntenic. This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.

Original languageEnglish (US)
Pages (from-to)355-369
Number of pages15
JournalSomatic Cell Genetics
Volume1
Issue number4
DOIs
StatePublished - Oct 1975

Fingerprint

Hybrid Cells
Cricetulus
Isoenzymes
Chromosomes
Prostaglandins D
Cell Separation
Chromosome Mapping
Spleen
Clone Cells
Growth

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes. / Minna, John D.; Marshall, Thomas H.; Shaffer-Berman, Patricia V.

In: Somatic Cell Genetics, Vol. 1, No. 4, 10.1975, p. 355-369.

Research output: Contribution to journalArticle

Minna, John D. ; Marshall, Thomas H. ; Shaffer-Berman, Patricia V. / Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes. In: Somatic Cell Genetics. 1975 ; Vol. 1, No. 4. pp. 355-369.
@article{a3dc55ad73304768a7d0d549af30da67,
title = "Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes",
abstract = "Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and one pair (PGD-PGM2) to be syntenic. This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.",
author = "Minna, {John D.} and Marshall, {Thomas H.} and Shaffer-Berman, {Patricia V.}",
year = "1975",
month = "10",
doi = "10.1007/BF01538667",
language = "English (US)",
volume = "1",
pages = "355--369",
journal = "Somatic Cell and Molecular Genetics",
issn = "0740-7750",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",
number = "4",

}

TY - JOUR

T1 - Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes

AU - Minna, John D.

AU - Marshall, Thomas H.

AU - Shaffer-Berman, Patricia V.

PY - 1975/10

Y1 - 1975/10

N2 - Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and one pair (PGD-PGM2) to be syntenic. This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.

AB - Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and one pair (PGD-PGM2) to be syntenic. This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.

UR - http://www.scopus.com/inward/record.url?scp=0016567973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016567973&partnerID=8YFLogxK

U2 - 10.1007/BF01538667

DO - 10.1007/BF01538667

M3 - Article

C2 - 1235911

AN - SCOPUS:0016567973

VL - 1

SP - 355

EP - 369

JO - Somatic Cell and Molecular Genetics

JF - Somatic Cell and Molecular Genetics

SN - 0740-7750

IS - 4

ER -