Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites

Shouzou Wei, James J. Brittin, J R Falck, Siddam Anjaiah, Kasem Nithipatikom, Lijie Cui, William B. Campbell, Jorge H. Capdevila

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalAnalytical biochemistry
Volume352
Issue number1
DOIs
StatePublished - May 1 2006

Keywords

  • Arachidonic acid epoxygenase
  • Chiral analysis
  • EETs
  • Endogenous EETs
  • Epoxyeicosatrienoic acids
  • P450 epoxygenase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Wei, S., Brittin, J. J., Falck, J. R., Anjaiah, S., Nithipatikom, K., Cui, L., Campbell, W. B., & Capdevila, J. H. (2006). Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites. Analytical biochemistry, 352(1), 129-134. https://doi.org/10.1016/j.ab.2006.01.005