Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites

Shouzou Wei; James J. Brittin; J R Falck; Siddam Anjaiah; Kasem Nithipatikom; Lijie Cui; William B. Campbell; Jorge H. Capdevila

doi:10.1016/j.ab.2006.01.005

Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites

Shouzou Wei, James J. Brittin, J R Falck, Siddam Anjaiah, Kasem Nithipatikom, Lijie Cui, William B. Campbell, Jorge H. Capdevila

Biochemistry

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.

Original language	English (US)
Pages (from-to)	129-134
Number of pages	6
Journal	Analytical biochemistry
Volume	352
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2006.01.005
State	Published - May 1 2006

Keywords

Arachidonic acid epoxygenase
Chiral analysis
EETs
Endogenous EETs
Epoxyeicosatrienoic acids
P450 epoxygenase

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.ab.2006.01.005

Cite this

@article{f78f43f456134d58af0453fd524cfc70,

title = "Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites",

abstract = "An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.",

keywords = "Arachidonic acid epoxygenase, Chiral analysis, EETs, Endogenous EETs, Epoxyeicosatrienoic acids, P450 epoxygenase",

author = "Shouzou Wei and Brittin, {James J.} and Falck, {J R} and Siddam Anjaiah and Kasem Nithipatikom and Lijie Cui and Campbell, {William B.} and Capdevila, {Jorge H.}",

note = "Funding Information: The support of the National Institute of General Medicine (37299 [J.H. Capdevila] and 31278 [J.R. Falck]), the National Heart, Lung, and Blood Institute (51055 [W.B. Campbell]), and the Robert A. Welch Foundation (J.R. Falck) is gratefully acknowledged.",

year = "2006",

month = may,

day = "1",

doi = "10.1016/j.ab.2006.01.005",

language = "English (US)",

volume = "352",

pages = "129--134",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites

AU - Wei, Shouzou

AU - Brittin, James J.

AU - Falck, J R

AU - Anjaiah, Siddam

AU - Nithipatikom, Kasem

AU - Cui, Lijie

AU - Campbell, William B.

AU - Capdevila, Jorge H.

N1 - Funding Information: The support of the National Institute of General Medicine (37299 [J.H. Capdevila] and 31278 [J.R. Falck]), the National Heart, Lung, and Blood Institute (51055 [W.B. Campbell]), and the Robert A. Welch Foundation (J.R. Falck) is gratefully acknowledged.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.

AB - An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.

KW - Arachidonic acid epoxygenase

KW - Chiral analysis

KW - EETs

KW - Endogenous EETs

KW - Epoxyeicosatrienoic acids

KW - P450 epoxygenase

UR - http://www.scopus.com/inward/record.url?scp=33646065988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646065988&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2006.01.005

DO - 10.1016/j.ab.2006.01.005

M3 - Article

C2 - 16480681

AN - SCOPUS:33646065988

SN - 0003-2697

VL - 352

SP - 129

EP - 134

JO - Analytical biochemistry

JF - Analytical biochemistry

IS - 1

ER -

Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this