TY - JOUR
T1 - Chitosan-silver nanoparticles as an approach to control bacterial proliferation, spores and antibiotic-resistant bacteria
AU - Alfaro-González, B.
AU - Ulate, D.
AU - Alvarado, R.
AU - Arguëllo-Miranda, O.
PY - 2018/3/9
Y1 - 2018/3/9
N2 - Nanoparticulate silver inhibits bacterial growth. Here we evaluated the antimicrobial properties of a novel form of silver nanoparticles, synthesized by a sonochemical method with chitosan as reducing and stabilizing agent (chitosan-silver nanoparticles). The chitosan-silver nanoparticles were positively charged and stable in the long-term, as determined by the high Z-potential. The minimum inhibitory concentration for vegetative bacteria and spores was measured with a resazurin microtiter assay. The interaction between nanoparticles and the bacterial surface was observed by atomic force microscopy and transmission electron microscopy. We found that chitosan-silver nanoparticles inhibited vegetative bacteria and bacterial spores. Furthermore, chitosan-silver nanoparticles enhanced the performance of ampicillin against bacteria and were also effective against an ampicillin-resistant strain. Atomic force microscopy and transmission electron microscopy images revealed a close interaction between the nanoparticles and bacterial extracellular structures, such as flagella. We suggest that chitosan-silver nanoparticles could be used as a topical antimicrobial that can enhance antibiotic performance.
AB - Nanoparticulate silver inhibits bacterial growth. Here we evaluated the antimicrobial properties of a novel form of silver nanoparticles, synthesized by a sonochemical method with chitosan as reducing and stabilizing agent (chitosan-silver nanoparticles). The chitosan-silver nanoparticles were positively charged and stable in the long-term, as determined by the high Z-potential. The minimum inhibitory concentration for vegetative bacteria and spores was measured with a resazurin microtiter assay. The interaction between nanoparticles and the bacterial surface was observed by atomic force microscopy and transmission electron microscopy. We found that chitosan-silver nanoparticles inhibited vegetative bacteria and bacterial spores. Furthermore, chitosan-silver nanoparticles enhanced the performance of ampicillin against bacteria and were also effective against an ampicillin-resistant strain. Atomic force microscopy and transmission electron microscopy images revealed a close interaction between the nanoparticles and bacterial extracellular structures, such as flagella. We suggest that chitosan-silver nanoparticles could be used as a topical antimicrobial that can enhance antibiotic performance.
KW - AFM
KW - antibiotic resistance
KW - chitosan-silver nanoparticles
KW - flagella
KW - sonochemical synthesis
KW - TEM,REMA
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U2 - 10.1088/2057-1976/aaaafe
DO - 10.1088/2057-1976/aaaafe
M3 - Article
AN - SCOPUS:85047244018
VL - 4
JO - Biomedical Physics and Engineering Express
JF - Biomedical Physics and Engineering Express
SN - 2057-1976
IS - 3
M1 - 035011
ER -