Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia

Peiwei Liu, Xiaochu Lou, Jenna L. Wingfield, Jianfeng Lin, Daniela Nicastro, Karl Lechtreck

Research output: Contribution to journalArticle

Abstract

Mutations in the channel protein PKD2 cause autosomal dominant polycystic kidney disease, but the function of PKD2 in cilia remains unclear. Here, we show that PKD2 targets and anchors mastigonemes, filamentous polymers of the glycoprotein MST1, to the extracellular surface of Chlamydomonas cilia. PKD2-mastigoneme complexes physically connect to the axonemal doublets 4 and 8, positioning them perpendicular to the plane of ciliary beating. pkd2 mutant cilia lack mastigonemes, and mutant cells swim with reduced velocity, indicating a motility-related function of the PKD2-mastigoneme complex. Association with both the axoneme and extracellular structures supports a mechanosensory role of Chlamydomonas PKD2. We propose that PKD2-mastigoneme arrays, on opposing sides of the cilium, could perceive forces during ciliary beating and transfer these signals to locally regulate the response of the axoneme.

Original languageEnglish (US)
Article number202001122
JournalJournal of Cell Biology
Volume219
Issue number6
DOIs
StatePublished - Jun 1 2020

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia'. Together they form a unique fingerprint.

  • Cite this