Chloroquine-induced lysosomal abnormalities in cultured foetal mouse hearts

Robert M. Ridout, Robert S. Decker, Kern Wildenthal

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Chloroquine (100 μm) induces abnormalities of lysosomal structure in cultured mouse hearts. After 1 or 2 days' exposure to the drug, large autophagic vacuoles with myeloid figures and inclusion bodies develop within myocytes and interstitial cells. Secondary lysosomes in interstitial cells occasionally contain cardiac myofilaments. In addition, after 2 days, the amounts of cathepsin D and β-acetylglucosaminidase decrease significantly, along with a significant redistribution of enzyme activity between sedimentable and non-sedimentable fractions. The results suggest that chloroquine is a useful experimental agent for causing lysosomal derangements in cardiac tissues under controlled conditions.

Original languageEnglish (US)
Pages (from-to)175-180,IN1-IN4,181-183
JournalJournal of Molecular and Cellular Cardiology
Volume10
Issue number2
DOIs
StatePublished - Feb 1978

Keywords

  • Acid phosphatase
  • Autophagy
  • Beta-acetylglucosaminidase
  • Cathepsin D
  • Chloroquine
  • Cytochemistry
  • Foetal mouse heart
  • Lysosomes
  • Organ culture

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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