Chloroquine-induced lysosomal abnormalities in cultured foetal mouse hearts

Robert M. Ridout, Robert S. Decker, Kern Wildenthal

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Chloroquine (100 μm) induces abnormalities of lysosomal structure in cultured mouse hearts. After 1 or 2 days' exposure to the drug, large autophagic vacuoles with myeloid figures and inclusion bodies develop within myocytes and interstitial cells. Secondary lysosomes in interstitial cells occasionally contain cardiac myofilaments. In addition, after 2 days, the amounts of cathepsin D and β-acetylglucosaminidase decrease significantly, along with a significant redistribution of enzyme activity between sedimentable and non-sedimentable fractions. The results suggest that chloroquine is a useful experimental agent for causing lysosomal derangements in cardiac tissues under controlled conditions.

Original languageEnglish (US)
JournalJournal of Molecular and Cellular Cardiology
Volume10
Issue number2
DOIs
StatePublished - 1978

Fingerprint

Fetal Heart
Chloroquine
Acetylglucosaminidase
Cathepsin D
Myofibrils
Inclusion Bodies
Vacuoles
Lysosomes
Muscle Cells
Enzymes
Pharmaceutical Preparations

Keywords

  • Acid phosphatase
  • Autophagy
  • Beta-acetylglucosaminidase
  • Cathepsin D
  • Chloroquine
  • Cytochemistry
  • Foetal mouse heart
  • Lysosomes
  • Organ culture

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Chloroquine-induced lysosomal abnormalities in cultured foetal mouse hearts. / Ridout, Robert M.; Decker, Robert S.; Wildenthal, Kern.

In: Journal of Molecular and Cellular Cardiology, Vol. 10, No. 2, 1978.

Research output: Contribution to journalArticle

Ridout, Robert M. ; Decker, Robert S. ; Wildenthal, Kern. / Chloroquine-induced lysosomal abnormalities in cultured foetal mouse hearts. In: Journal of Molecular and Cellular Cardiology. 1978 ; Vol. 10, No. 2.
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