Cholecystokinin-octapeptide fragments: Binding to brain cholecystokinin receptors

Martha Knight, Carol A. Tamminga, Luca Steardo, Mary E. Beck, Paolo Barone, Thomas N. Chase

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Structural determinants of cholecystokinin octapeptide (CCK-8) binding to central nervous system receptors have been studied to assess the relative importance of the amino and the carboxyl end of the active peptide sequence, CCK-(26-33). The relative ability to inhibit equilibrium binding of [125I]CCK-33 to guinea pig cortical membranes was determined for a series of amino and carboxyl terminal fragments of CCK-8. While N-acetyl CCK-(26-29), N-acetyl CCK-(26-30) amide and N-acetyl CCK-(26-31) amide were inactive, the N-acetyl CCK-(26-32) amide fragment displayed binding to central receptors. Of the carboxyl terminal peptide fragments, both CCK-(29-33) and CCK-(30-33) bound less potently than CCK-8; CCK-(31-33) interacted more weakly than the tetra- and pentapeptide, but with a higher affinity to brain receptors than to peripheral receptors. The heptapeptide, CCK-(26-32) amide, and the tripeptide, CCK-(31-33), are known to antagonize CCK action at peripheral receptors. The heptapeptide bound to central receptors 25 times more potently than a known peripheral antagonist, dibutyryl cyclic GMP. Thus these peptides may act centrally to oppose CCK-8 mediated functions.

Original languageEnglish (US)
Pages (from-to)49-55
Number of pages7
JournalEuropean Journal of Pharmacology
Issue number1-2
StatePublished - Oct 1 1984


  • Brain receptors
  • CCK-5, CCK-4, CCK-3
  • Cholecystokinin antagonists
  • Cholecystokinin fragments
  • Cholecystokinin receptors
  • Guinea pig cortical membrane
  • N-terminal CCK fragment peptides

ASJC Scopus subject areas

  • Pharmacology


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