Cholesterol crystallization in model biles

Effects of bile salt and phospholipid species composition

Antonio Moschetta, Gerard P. VanBerge-Henegouwen, Piero Portincasa, Giuseppe Palasciano, Karel J. Van Erpecum

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cholesterol in human bile is solubilized in micelles by (relatively hydrophobic) bile salts and phosphatidylcholine (unsaturated acyl chains at sn-2 position). Hydrophilic tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all decrease cholesterol crystal-containing zones in the equilibrium ternary phase diagram (van Erpecum, K. J., and M. C. Carey. 1997. Biochim. Biophys. Acta. 1345: 269-282) and thus could be valuable in gallstone prevention. We have now compared crystallization in cholesterol-supersaturated model systems (3.6 g/dl, 37°C) composed of various bile salts as well as egg yolk phosphatidylcholine (unsaturated acyl chains at sn-2 position), dipalmitoyl phosphatidylcholine, or sphingomyelin throughout the phase diagram. At low phospholipid contents [left two-phase (micelle plus crystal-containing) zone], tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all enhanced crystallization. At pathophysiologically relevant intermediate phospholipid contents [central three-phase (micelle plus vesicle plus crystal-containing) zone], tauroursodeoxycholate inhibited, but dipalmitoyl phosphatidylcholine and sphingomyelin enhanced, crystallization. Also, during 10 days of incubation, there was a strong decrease in vesicular cholesterol contents and vesicular cholesterol-to-phospholipid ratios (∼1 on day 10), coinciding with a strong increase in crystal mass. At high phospholipid contents [right two-phase (micelle plus vesicle-containing) zone], vesicles were always unsaturated and crystallization did not occur. Strategies aiming to increase amounts of hydrophilic bile salts may be preferable to increasing saturated phospholipids in bile, because the latter may enhance crystallization.

Original languageEnglish (US)
Pages (from-to)1273-1281
Number of pages9
JournalJournal of Lipid Research
Volume42
Issue number8
StatePublished - 2001

Fingerprint

1,2-Dipalmitoylphosphatidylcholine
Crystallization
Bile Acids and Salts
Bile
Sphingomyelins
Micelles
Phospholipids
Cholesterol
Chemical analysis
Crystals
Phosphatidylcholines
Phase diagrams
Egg Yolk
Gallstones
tauroursodeoxycholic acid

Keywords

  • Crystals
  • Dialysis
  • Intermixed micellar/vesicular bile salt concentration
  • Micelles
  • Phosphatidylcholine
  • Sphingomyelin
  • Taurocholate
  • Taurodeoxycholate
  • Tauroursodeoxycholate
  • Ultrafiltration
  • Vesicles

ASJC Scopus subject areas

  • Endocrinology

Cite this

Moschetta, A., VanBerge-Henegouwen, G. P., Portincasa, P., Palasciano, G., & Van Erpecum, K. J. (2001). Cholesterol crystallization in model biles: Effects of bile salt and phospholipid species composition. Journal of Lipid Research, 42(8), 1273-1281.

Cholesterol crystallization in model biles : Effects of bile salt and phospholipid species composition. / Moschetta, Antonio; VanBerge-Henegouwen, Gerard P.; Portincasa, Piero; Palasciano, Giuseppe; Van Erpecum, Karel J.

In: Journal of Lipid Research, Vol. 42, No. 8, 2001, p. 1273-1281.

Research output: Contribution to journalArticle

Moschetta, A, VanBerge-Henegouwen, GP, Portincasa, P, Palasciano, G & Van Erpecum, KJ 2001, 'Cholesterol crystallization in model biles: Effects of bile salt and phospholipid species composition', Journal of Lipid Research, vol. 42, no. 8, pp. 1273-1281.
Moschetta A, VanBerge-Henegouwen GP, Portincasa P, Palasciano G, Van Erpecum KJ. Cholesterol crystallization in model biles: Effects of bile salt and phospholipid species composition. Journal of Lipid Research. 2001;42(8):1273-1281.
Moschetta, Antonio ; VanBerge-Henegouwen, Gerard P. ; Portincasa, Piero ; Palasciano, Giuseppe ; Van Erpecum, Karel J. / Cholesterol crystallization in model biles : Effects of bile salt and phospholipid species composition. In: Journal of Lipid Research. 2001 ; Vol. 42, No. 8. pp. 1273-1281.
@article{da012086032f43fc9964d55fda72e9ff,
title = "Cholesterol crystallization in model biles: Effects of bile salt and phospholipid species composition",
abstract = "Cholesterol in human bile is solubilized in micelles by (relatively hydrophobic) bile salts and phosphatidylcholine (unsaturated acyl chains at sn-2 position). Hydrophilic tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all decrease cholesterol crystal-containing zones in the equilibrium ternary phase diagram (van Erpecum, K. J., and M. C. Carey. 1997. Biochim. Biophys. Acta. 1345: 269-282) and thus could be valuable in gallstone prevention. We have now compared crystallization in cholesterol-supersaturated model systems (3.6 g/dl, 37°C) composed of various bile salts as well as egg yolk phosphatidylcholine (unsaturated acyl chains at sn-2 position), dipalmitoyl phosphatidylcholine, or sphingomyelin throughout the phase diagram. At low phospholipid contents [left two-phase (micelle plus crystal-containing) zone], tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all enhanced crystallization. At pathophysiologically relevant intermediate phospholipid contents [central three-phase (micelle plus vesicle plus crystal-containing) zone], tauroursodeoxycholate inhibited, but dipalmitoyl phosphatidylcholine and sphingomyelin enhanced, crystallization. Also, during 10 days of incubation, there was a strong decrease in vesicular cholesterol contents and vesicular cholesterol-to-phospholipid ratios (∼1 on day 10), coinciding with a strong increase in crystal mass. At high phospholipid contents [right two-phase (micelle plus vesicle-containing) zone], vesicles were always unsaturated and crystallization did not occur. Strategies aiming to increase amounts of hydrophilic bile salts may be preferable to increasing saturated phospholipids in bile, because the latter may enhance crystallization.",
keywords = "Crystals, Dialysis, Intermixed micellar/vesicular bile salt concentration, Micelles, Phosphatidylcholine, Sphingomyelin, Taurocholate, Taurodeoxycholate, Tauroursodeoxycholate, Ultrafiltration, Vesicles",
author = "Antonio Moschetta and VanBerge-Henegouwen, {Gerard P.} and Piero Portincasa and Giuseppe Palasciano and {Van Erpecum}, {Karel J.}",
year = "2001",
language = "English (US)",
volume = "42",
pages = "1273--1281",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "8",

}

TY - JOUR

T1 - Cholesterol crystallization in model biles

T2 - Effects of bile salt and phospholipid species composition

AU - Moschetta, Antonio

AU - VanBerge-Henegouwen, Gerard P.

AU - Portincasa, Piero

AU - Palasciano, Giuseppe

AU - Van Erpecum, Karel J.

PY - 2001

Y1 - 2001

N2 - Cholesterol in human bile is solubilized in micelles by (relatively hydrophobic) bile salts and phosphatidylcholine (unsaturated acyl chains at sn-2 position). Hydrophilic tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all decrease cholesterol crystal-containing zones in the equilibrium ternary phase diagram (van Erpecum, K. J., and M. C. Carey. 1997. Biochim. Biophys. Acta. 1345: 269-282) and thus could be valuable in gallstone prevention. We have now compared crystallization in cholesterol-supersaturated model systems (3.6 g/dl, 37°C) composed of various bile salts as well as egg yolk phosphatidylcholine (unsaturated acyl chains at sn-2 position), dipalmitoyl phosphatidylcholine, or sphingomyelin throughout the phase diagram. At low phospholipid contents [left two-phase (micelle plus crystal-containing) zone], tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all enhanced crystallization. At pathophysiologically relevant intermediate phospholipid contents [central three-phase (micelle plus vesicle plus crystal-containing) zone], tauroursodeoxycholate inhibited, but dipalmitoyl phosphatidylcholine and sphingomyelin enhanced, crystallization. Also, during 10 days of incubation, there was a strong decrease in vesicular cholesterol contents and vesicular cholesterol-to-phospholipid ratios (∼1 on day 10), coinciding with a strong increase in crystal mass. At high phospholipid contents [right two-phase (micelle plus vesicle-containing) zone], vesicles were always unsaturated and crystallization did not occur. Strategies aiming to increase amounts of hydrophilic bile salts may be preferable to increasing saturated phospholipids in bile, because the latter may enhance crystallization.

AB - Cholesterol in human bile is solubilized in micelles by (relatively hydrophobic) bile salts and phosphatidylcholine (unsaturated acyl chains at sn-2 position). Hydrophilic tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all decrease cholesterol crystal-containing zones in the equilibrium ternary phase diagram (van Erpecum, K. J., and M. C. Carey. 1997. Biochim. Biophys. Acta. 1345: 269-282) and thus could be valuable in gallstone prevention. We have now compared crystallization in cholesterol-supersaturated model systems (3.6 g/dl, 37°C) composed of various bile salts as well as egg yolk phosphatidylcholine (unsaturated acyl chains at sn-2 position), dipalmitoyl phosphatidylcholine, or sphingomyelin throughout the phase diagram. At low phospholipid contents [left two-phase (micelle plus crystal-containing) zone], tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all enhanced crystallization. At pathophysiologically relevant intermediate phospholipid contents [central three-phase (micelle plus vesicle plus crystal-containing) zone], tauroursodeoxycholate inhibited, but dipalmitoyl phosphatidylcholine and sphingomyelin enhanced, crystallization. Also, during 10 days of incubation, there was a strong decrease in vesicular cholesterol contents and vesicular cholesterol-to-phospholipid ratios (∼1 on day 10), coinciding with a strong increase in crystal mass. At high phospholipid contents [right two-phase (micelle plus vesicle-containing) zone], vesicles were always unsaturated and crystallization did not occur. Strategies aiming to increase amounts of hydrophilic bile salts may be preferable to increasing saturated phospholipids in bile, because the latter may enhance crystallization.

KW - Crystals

KW - Dialysis

KW - Intermixed micellar/vesicular bile salt concentration

KW - Micelles

KW - Phosphatidylcholine

KW - Sphingomyelin

KW - Taurocholate

KW - Taurodeoxycholate

KW - Tauroursodeoxycholate

KW - Ultrafiltration

KW - Vesicles

UR - http://www.scopus.com/inward/record.url?scp=0034884788&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034884788&partnerID=8YFLogxK

M3 - Article

VL - 42

SP - 1273

EP - 1281

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 8

ER -