Cholesterol synthesis by human fetal hepatocytes: Effect of lipoproteins

B. R. Carr, E. R. Simpson

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The purpose of the present investigation was to determine the effect of various lipoproteins on the rate of cholesterol synthesis of human fetal liver cells maintained in culture. This was accomplished by measuring the rate of incorporation of tritium from tritiated water or carbon 14-labeled acetate into cholesterol in human fetal liver cells. Optimal conditions for each assay were determined. When human fetal liver cells were maintained in the presence of low-density lipoprotein, cholesterol synthesis was inhibited in a concentration-dependent fashion. Intermediate—density lipoprotein and very-low-density lipoprotein also suppressed cholesterol synthesis in human fetal liver cells. In contrast, high-density lipoprotein stimulated cholesterol synthesis in human fetal liver cells. The results of the present as well as our previous investigations suggest that multiple interrelationships exist between fetal liver cholesterol synthesis and lipoprotein-cholesterol utilization by the human fetal adrenal gland and that these processes serve to regulate the lipoprotein-cholesterol levels in fetal plasma. (Am J Obstet Gynecol 1984;150:551-7.)

Original languageEnglish (US)
Pages (from-to)551-557
Number of pages7
JournalAmerican journal of obstetrics and gynecology
Volume150
Issue number5
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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