Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells

Etienne Morel, Sara Ghezzal, Géraldine Lucchi, Caroline Truntzer, Jean Paul Pais de Barros, Françoise Simon-Plas, Sylvie Demignot, Chieko Mineo, Philip W. Shaul, Armelle Leturque, Monique Rousset, Véronique Carrière

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Scavenger receptor Class B type 1 (SR-B1) is a lipid transporter and sensor. In intestinal epithelial cells, SR-B1-dependent lipid sensing is associated with SR-B1 recruitment in raft-like/ detergent-resistant membrane domains and interaction of its C-terminal transmembrane domain with plasma membrane cholesterol. To clarify the initiating events occurring during lipid sensing by SR-B1, we analyzed cholesterol trafficking and raft-like domain composition in intestinal epithelial cells expressing wild-type SR-B1 or the mutated form SR-B1-Q445A, defective in membrane cholesterol binding and signal initiation. These features of SR-B1 were found to influence both apical cholesterol efflux and intracellular cholesterol trafficking from plasma membrane to lipid droplets, and the lipid composition of raft-like domains. Lipidomic analysis revealed likely participation of d18:0/16:0 sphingomyelin and 16:0/0:0 lysophosphatidylethanolamine in lipid sensing by SR-B1. Proteomic analysis identified proteins, whose abundance changed in raft-like domains during lipid sensing, and these included molecules linked to lipid raft dynamics and signal transduction. These findings provide new insights into the role of SR-B1 in cellular cholesterol homeostasis and suggest molecular links between SR-B1-dependent lipid sensing and cell cholesterol and lipid droplet dynamics.

Original languageEnglish (US)
Pages (from-to)199-211
Number of pages13
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1863
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

CD36 Antigens
Epithelial Cells
Cholesterol
Lipids
Membranes
Cell Membrane
Sphingomyelins
Membrane Lipids
Detergents
Proteomics
Signal Transduction
Homeostasis

Keywords

  • Cholesterol
  • Lipid raft
  • Lipid trafficking
  • Scavenger receptor
  • Sphingolipid

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells. / Morel, Etienne; Ghezzal, Sara; Lucchi, Géraldine; Truntzer, Caroline; Pais de Barros, Jean Paul; Simon-Plas, Françoise; Demignot, Sylvie; Mineo, Chieko; Shaul, Philip W.; Leturque, Armelle; Rousset, Monique; Carrière, Véronique.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1863, No. 2, 01.02.2018, p. 199-211.

Research output: Contribution to journalArticle

Morel, Etienne ; Ghezzal, Sara ; Lucchi, Géraldine ; Truntzer, Caroline ; Pais de Barros, Jean Paul ; Simon-Plas, Françoise ; Demignot, Sylvie ; Mineo, Chieko ; Shaul, Philip W. ; Leturque, Armelle ; Rousset, Monique ; Carrière, Véronique. / Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells. In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2018 ; Vol. 1863, No. 2. pp. 199-211.
@article{e6fa79f06dcd4c84a22becbed520e506,
title = "Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells",
abstract = "Scavenger receptor Class B type 1 (SR-B1) is a lipid transporter and sensor. In intestinal epithelial cells, SR-B1-dependent lipid sensing is associated with SR-B1 recruitment in raft-like/ detergent-resistant membrane domains and interaction of its C-terminal transmembrane domain with plasma membrane cholesterol. To clarify the initiating events occurring during lipid sensing by SR-B1, we analyzed cholesterol trafficking and raft-like domain composition in intestinal epithelial cells expressing wild-type SR-B1 or the mutated form SR-B1-Q445A, defective in membrane cholesterol binding and signal initiation. These features of SR-B1 were found to influence both apical cholesterol efflux and intracellular cholesterol trafficking from plasma membrane to lipid droplets, and the lipid composition of raft-like domains. Lipidomic analysis revealed likely participation of d18:0/16:0 sphingomyelin and 16:0/0:0 lysophosphatidylethanolamine in lipid sensing by SR-B1. Proteomic analysis identified proteins, whose abundance changed in raft-like domains during lipid sensing, and these included molecules linked to lipid raft dynamics and signal transduction. These findings provide new insights into the role of SR-B1 in cellular cholesterol homeostasis and suggest molecular links between SR-B1-dependent lipid sensing and cell cholesterol and lipid droplet dynamics.",
keywords = "Cholesterol, Lipid raft, Lipid trafficking, Scavenger receptor, Sphingolipid",
author = "Etienne Morel and Sara Ghezzal and G{\'e}raldine Lucchi and Caroline Truntzer and {Pais de Barros}, {Jean Paul} and Fran{\cc}oise Simon-Plas and Sylvie Demignot and Chieko Mineo and Shaul, {Philip W.} and Armelle Leturque and Monique Rousset and V{\'e}ronique Carri{\`e}re",
year = "2018",
month = "2",
day = "1",
doi = "10.1016/j.bbalip.2017.11.009",
language = "English (US)",
volume = "1863",
pages = "199--211",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells

AU - Morel, Etienne

AU - Ghezzal, Sara

AU - Lucchi, Géraldine

AU - Truntzer, Caroline

AU - Pais de Barros, Jean Paul

AU - Simon-Plas, Françoise

AU - Demignot, Sylvie

AU - Mineo, Chieko

AU - Shaul, Philip W.

AU - Leturque, Armelle

AU - Rousset, Monique

AU - Carrière, Véronique

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Scavenger receptor Class B type 1 (SR-B1) is a lipid transporter and sensor. In intestinal epithelial cells, SR-B1-dependent lipid sensing is associated with SR-B1 recruitment in raft-like/ detergent-resistant membrane domains and interaction of its C-terminal transmembrane domain with plasma membrane cholesterol. To clarify the initiating events occurring during lipid sensing by SR-B1, we analyzed cholesterol trafficking and raft-like domain composition in intestinal epithelial cells expressing wild-type SR-B1 or the mutated form SR-B1-Q445A, defective in membrane cholesterol binding and signal initiation. These features of SR-B1 were found to influence both apical cholesterol efflux and intracellular cholesterol trafficking from plasma membrane to lipid droplets, and the lipid composition of raft-like domains. Lipidomic analysis revealed likely participation of d18:0/16:0 sphingomyelin and 16:0/0:0 lysophosphatidylethanolamine in lipid sensing by SR-B1. Proteomic analysis identified proteins, whose abundance changed in raft-like domains during lipid sensing, and these included molecules linked to lipid raft dynamics and signal transduction. These findings provide new insights into the role of SR-B1 in cellular cholesterol homeostasis and suggest molecular links between SR-B1-dependent lipid sensing and cell cholesterol and lipid droplet dynamics.

AB - Scavenger receptor Class B type 1 (SR-B1) is a lipid transporter and sensor. In intestinal epithelial cells, SR-B1-dependent lipid sensing is associated with SR-B1 recruitment in raft-like/ detergent-resistant membrane domains and interaction of its C-terminal transmembrane domain with plasma membrane cholesterol. To clarify the initiating events occurring during lipid sensing by SR-B1, we analyzed cholesterol trafficking and raft-like domain composition in intestinal epithelial cells expressing wild-type SR-B1 or the mutated form SR-B1-Q445A, defective in membrane cholesterol binding and signal initiation. These features of SR-B1 were found to influence both apical cholesterol efflux and intracellular cholesterol trafficking from plasma membrane to lipid droplets, and the lipid composition of raft-like domains. Lipidomic analysis revealed likely participation of d18:0/16:0 sphingomyelin and 16:0/0:0 lysophosphatidylethanolamine in lipid sensing by SR-B1. Proteomic analysis identified proteins, whose abundance changed in raft-like domains during lipid sensing, and these included molecules linked to lipid raft dynamics and signal transduction. These findings provide new insights into the role of SR-B1 in cellular cholesterol homeostasis and suggest molecular links between SR-B1-dependent lipid sensing and cell cholesterol and lipid droplet dynamics.

KW - Cholesterol

KW - Lipid raft

KW - Lipid trafficking

KW - Scavenger receptor

KW - Sphingolipid

UR - http://www.scopus.com/inward/record.url?scp=85044727955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044727955&partnerID=8YFLogxK

U2 - 10.1016/j.bbalip.2017.11.009

DO - 10.1016/j.bbalip.2017.11.009

M3 - Article

C2 - 29196159

AN - SCOPUS:85044727955

VL - 1863

SP - 199

EP - 211

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 2

ER -