Chromatin associated Sin3A is essential for male germ cell lineage in the mouse

Jessica Pellegrino, Diego H. Castrillon, Gregory David

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Spermatogenesis is a complex process that requires coordinated proliferation and differentiation of male germ cells. The molecular events that dictate this process are largely unknown, but are likely to involve highly regulated transcriptional control. In this study, we investigate the contribution of chromatin associated Sin3A in mouse germ cell lineage development. Genetic inactivation of Sin3A in the male germline leads to sterility that results from the early and penetrant apoptotic death observed in Sin3A-deleted germ cells, coincident with the reentry in mitosis. Sin3A-deleted testes exhibit a Sertoli-cell only phenotype, consistent with the absolute requirement for Sin3A in germ cells' development and/or viability. Interestingly, transcripts analysis revealed that the expression program of Sertoli cells is altered upon inactivation of Sin3A in germ cells. These studies identified a central role for the mammalian Sin3-HDAC complex in the germ cell lineage, and point to an exquisite transcriptional crosstalk between germ cells and their niche to support fertility in mammals.

Original languageEnglish (US)
Pages (from-to)349-355
Number of pages7
JournalDevelopmental Biology
Volume369
Issue number2
DOIs
StatePublished - Sep 15 2012

Keywords

  • Germ cells
  • Knock-out mouse
  • Sertoli cells
  • Sin3A
  • Testis
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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