Chromatin remodelers fine-tune h3k36me-directed deacetylation of neighbor nucleosomes by Rpd3S

Chul Hwan Lee, Jun Wu, Bing Li

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Chromatin remodelers have been implicated in the regulation of histone-modifying complexes. However, the underlying mechanism remains poorly understood. The Rpd3S histone deacetylase complex is recruited by elongating RNA polymerase II to remove histone acetylation at coding regions in a manner that is dependent on methylation of lysine 36 on histone 3 (H3K36me), and Rpd3S prefers dinucleosomes. Here, we show that the binding of Rpd3S to dinucleosomes and its catalytic activity are sensitive to the length of nucleosomal linker in a nonlinear fashion. Intriguingly, we found that H3K36me on one nucleosome stimulates Rpd3S to deacetylate the neighboring nucleosomes when those two nucleosomes are within an optimal distance. Finally, we demonstrate that chromatin remodelers enhance Rpd3S activity by altering nucleosomal spacing, suggesting that chromatin remodelers prime chromatin configuration to fine-tune subsequent histone modification reactions. This mechanism is important for accurate temporal control of chromatin dynamics during the transcription elongation cycle.

Original languageEnglish (US)
Pages (from-to)255-263
Number of pages9
JournalMolecular Cell
Volume52
Issue number2
DOIs
StatePublished - Oct 24 2013

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Nucleosomes
Chromatin
Histones
Histone Code
Histone Deacetylases
RNA Polymerase II
Acetylation
Methylation
Lysine

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Chromatin remodelers fine-tune h3k36me-directed deacetylation of neighbor nucleosomes by Rpd3S. / Lee, Chul Hwan; Wu, Jun; Li, Bing.

In: Molecular Cell, Vol. 52, No. 2, 24.10.2013, p. 255-263.

Research output: Contribution to journalArticle

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