Chromatography of Ac-Asp-Tyr-Met-Gly-Trp-Met-Asp-Nh, on The Horizontal Flow-Through Coil Planet Centrifuge and The High-Speed Multi-Layer Coil Planet Centrifuge

M. Knight, Y. Ito, A. M. Kask, C. A. Tamminga, T. N. Chase

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The peptide Ac-Asp-Tyr-Met-Gly-Trp-Met-Asp-NHp was purified by countercurrent chromatography in the horizontal flow-through coil planet centrifuge. The solvent system used was ammonium acetate, pH 8.5 and n-butanol (1:1 by volume). The high pH served to maintain the peptide in solution. When the upper phase was utilized as the mobile phase better separation of the peptide from impurities resulted. The peptide was also chromatographed in a new apparatus, the high-speed multi-layer coil planet centrifuge. With the lower phase mobile and at a higher temperature, the peptide was fractionated very rapidly in 30 min compared to 7 hr on the other instrument.

Original languageEnglish (US)
Pages (from-to)2525-2533
Number of pages9
JournalJournal of Liquid Chromatography
Volume7
Issue number13
DOIs
StatePublished - Nov 1 1984

ASJC Scopus subject areas

  • Molecular Medicine

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