Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase

N. K. Honey, A. Y. Sakaguchi, C. Quinto, R. J. MacDonald, G. I. Bell, C. Craik, W. J. Rutter, S. L. Naylor

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The genes for the serine proteases trypsin, chymotrypsin B, and elastase were chromosomally assigned in man using cDNA probes that have been isolated from a rat pancreatic cDNA library. DNA from human × rodent somatic cell hybrids was cleaved with BamHI or EcoRI and analyzed by Southern filter hybridization methods for the segregation of the genes for trypsin-1 (TRY1), chymotrypsin B (CTRB), and elastase-1 (ELA1). TRY1 was assigned to human chromosome 7q22→qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalSomatic Cell and Molecular Genetics
Volume10
Issue number4
DOIs
StatePublished - Jul 1984

Fingerprint

Pancreatic Elastase
Serine Proteases
Trypsin
Genes
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 12
Hybrid Cells
Human Chromosomes
Gene Library
Rodentia
Complementary DNA
Chromosomes
chymotrypsin B
DNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase. / Honey, N. K.; Sakaguchi, A. Y.; Quinto, C.; MacDonald, R. J.; Bell, G. I.; Craik, C.; Rutter, W. J.; Naylor, S. L.

In: Somatic Cell and Molecular Genetics, Vol. 10, No. 4, 07.1984, p. 369-376.

Research output: Contribution to journalArticle

Honey, NK, Sakaguchi, AY, Quinto, C, MacDonald, RJ, Bell, GI, Craik, C, Rutter, WJ & Naylor, SL 1984, 'Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase', Somatic Cell and Molecular Genetics, vol. 10, no. 4, pp. 369-376. https://doi.org/10.1007/BF01535632
Honey, N. K. ; Sakaguchi, A. Y. ; Quinto, C. ; MacDonald, R. J. ; Bell, G. I. ; Craik, C. ; Rutter, W. J. ; Naylor, S. L. / Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase. In: Somatic Cell and Molecular Genetics. 1984 ; Vol. 10, No. 4. pp. 369-376.
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