Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites

Peter N. Cockerill, William T. Garrard

Research output: Contribution to journalArticlepeer-review

732 Scopus citations

Abstract

Introduction of torsional stress into active chromatin domains requires that linear DNA molecules be anchored in vivo to impede free rotation. While searching for these anchorage elements, we have localized a nuclear matrix association region (MAR) within the mouse immunoglobulin x gene that contains two topoisomerase II sites and is adjacent to the tissue-specific enhancer. The same matrix contact occurs when the x locus is in germ-line (inactive) or rear-ranged (transcribed) configurations. This constitutive anchorage site partitions the gene into V-J and C region chromatin domains. We demonstrate that at least 10,000 similar and evolutionarily conserved MAR binding sites exist in the nucleus. We propose that these sites, in association with topoisomerase II and possibly in conjunction with enhancers, play fundamental roles in the functional organization of chromatin loop domains.

Original languageEnglish (US)
Pages (from-to)273-282
Number of pages10
JournalCell
Volume44
Issue number2
DOIs
StatePublished - Jan 31 1986

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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