Chromosome end-to-end associations and telomerase activity during cancer progression in human cells after treatment with α-particles simulating radon progeny

Tej K. Pandita, Eric J. Hall, Tom K. Hei, Mieczyslaw A. Piatyszek, Woodring E. Wright, Chang Q. Piao, Raj K. Pandita, James C. Willey, Charles R. Geard, Michael B. Kastan, Jerry W. Shay

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Chromosome end-to-end associations seen at metaphase involve telomeres and are commonly observed in cells derived from individuals with ataxia telangiectasia and most types of human tumors. The associations may arise because of short telomeres and/or alterations of chromatin structure. There is a growing consensus that telomere length is stabilized by the activity of telomerase in immortal cells; however, it is not clear why some immortal cells display chromosome end-to-end associations. In the present study me evaluated chromosome end-to-end associations, telomere length and telomerase activity with the tumorigenic status of human bronchial epithelial cells immortalized with human papillomavirus. Oncogenic transformation was initiated using radon simulated α-particles and cells evaluated as primary, secondary and metastatic transformants. The fewest chromosome end associations and lowest telomerase activity were observed in the parental immortalized cells. However, increased levels of telomerase activity were detected in α-particle survivors while robust telomerase activity was seen in the tumorigenic cell lines, The tumorigenic cells that were telomerase positive and had the highest frequency of cells with chromosome end-to-end associations were also metastatic. No correlation was found between telomere length and the different stages of carcinogenicity.

Original languageEnglish (US)
Pages (from-to)1423-1430
Number of pages8
JournalOncogene
Volume13
Issue number7
StatePublished - 1996

Fingerprint

Radon
Telomerase
Telomere
Chromosomes
Neoplasms
Ataxia Telangiectasia
Metaphase
Chromatin
Epithelial Cells
Cell Line

Keywords

  • AT
  • Metastasis
  • p53
  • Radiations
  • Telomeres
  • Tumors

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Chromosome end-to-end associations and telomerase activity during cancer progression in human cells after treatment with α-particles simulating radon progeny. / Pandita, Tej K.; Hall, Eric J.; Hei, Tom K.; Piatyszek, Mieczyslaw A.; Wright, Woodring E.; Piao, Chang Q.; Pandita, Raj K.; Willey, James C.; Geard, Charles R.; Kastan, Michael B.; Shay, Jerry W.

In: Oncogene, Vol. 13, No. 7, 1996, p. 1423-1430.

Research output: Contribution to journalArticle

Pandita, TK, Hall, EJ, Hei, TK, Piatyszek, MA, Wright, WE, Piao, CQ, Pandita, RK, Willey, JC, Geard, CR, Kastan, MB & Shay, JW 1996, 'Chromosome end-to-end associations and telomerase activity during cancer progression in human cells after treatment with α-particles simulating radon progeny', Oncogene, vol. 13, no. 7, pp. 1423-1430.
Pandita, Tej K. ; Hall, Eric J. ; Hei, Tom K. ; Piatyszek, Mieczyslaw A. ; Wright, Woodring E. ; Piao, Chang Q. ; Pandita, Raj K. ; Willey, James C. ; Geard, Charles R. ; Kastan, Michael B. ; Shay, Jerry W. / Chromosome end-to-end associations and telomerase activity during cancer progression in human cells after treatment with α-particles simulating radon progeny. In: Oncogene. 1996 ; Vol. 13, No. 7. pp. 1423-1430.
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