Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases

Yuhong Han, Jin Jiang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Hedgehog (Hh) signaling culminates in the conversion of the latent transcription factor Cubitus interruptus (Ci)/Gli from a repressor form (CiR/GliR) into an activator form (CiA/GliA). While sequential phosphorylation of Ci/Gli by protein kinase A(PKA), glycogen synthase kinase 3 (GSK3), and casein kinase 1 (CK1) is essential for its proteolytic processing that generates CiR/GliR, sequential phosphorylation of Ci/Gli by the Fused (Fu)/Unc-51 like kinase (Ulk) family kinases Fu/Ulk3/Stk36 and CK1 contributes to the formation of CiA/GliA. Fu/Ulk3/Stk36-mediated phosphorylation of Ci/Gli is stimulated by Hh, leading to altered interaction between Ci/Gli and the Hh pathway repressor Sufu. Here we describe both in vitro and in vivo assays that determine Ci/Gli phosphorylation by the Fu/Ulk family kinases and its regulation by Hh.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages213-229
Number of pages17
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2374
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Atg1
  • CK1
  • Ci
  • Fu
  • Gli1
  • Gli2
  • Gli3
  • Hh
  • Kinase
  • Phosphorylation
  • Shh
  • Signaling
  • Stk36
  • Ulk1
  • Ulk3

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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