Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating

Paulina Urbanska, Ewa Joachimiak, Rafał Bazan, Gang Fu, Martyna Poprzeczko, Hanna Fabczak, Daniela Nicastro, Dorota Wloga

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Cilia beating is powered by the inner and outer dynein arms (IDAs and ODAs). These multi-subunit macrocomplexes are arranged in two rows on each outer doublet along the entire cilium length, except its distal end. To generate cilia beating, the activity of ODAs and IDAs must be strictly regulated locally by interactions with the dynein arm-associated structures within each ciliary unit and coordinated globally in time and space between doublets and along the axoneme. Here, we provide evidence of a novel ciliary complex composed of two conserved WD-repeat proteins, Fap43p and Fap44p. This complex is adjacent to another WD-repeat protein, Fap57p, and most likely the two-headed inner dynein arm, IDA I1. Loss of either protein results in altered waveform, beat stroke and reduced swimming speed. The ciliary localization of Fap43p and Fap44p is interdependent in the ciliate Tetrahymena thermophila.

Original languageEnglish (US)
Pages (from-to)4479-4493
Number of pages15
JournalCellular and Molecular Life Sciences
Volume75
Issue number24
DOIs
StatePublished - Dec 1 2018

Keywords

  • Dyh6
  • Dyh7
  • Tether/tether head complex
  • Wdr52
  • Wdr65
  • Wdr96

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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