Circadian and CLOCK-controlled regulation of the mouse transcriptome and cell proliferation

Brooke H. Miller, Erin L. McDearmon, Satchidananda Panda, Kevin R. Hayes, Jie Zhang, Jessica L. Andrews, Marina P. Antoch, John R. Walker, Karyn A. Esser, John B. Hogenesch, Joseph S. Takahashi

Research output: Contribution to journalArticle

306 Citations (Scopus)

Abstract

Circadian rhythms of cell and organismal physiology are controlled by an autoregulatory transcription-translation feedback loop that regulates the expression of rhythmic genes in a tissue-specific manner. Recent studies have suggested that components of the circadian pacemaker, such as the Clock and Per2 gene products, regulate a wide variety of processes, including obesity, sensitization to cocaine, cancer susceptibility, and morbidity to chemotherapeutic agents. To identify a more complete cohort of genes that are transcriptionally regulated by CLOCK and/or circadian rhythms, we used a DNA array interrogating the mouse protein-encoding transcriptome to measure gene expression in liver and skeletal muscle from WT and Clock mutant mice. In WT tissue, we found that a large percentage of expressed genes were transcription factors that were rhythmic in either muscle or liver, but not in both, suggesting that tissue-specific output of the pacemaker is regulated in part by a transcriptional cascade. In comparing tissues from WT and Clock mutant mice, we found that the Clock mutation affects the expression of many genes that are rhythmic in WT tissue, but also profoundly affects many nonrhythmic genes. In both liver and skeletal muscle, a significant number of CLOCK-regulated genes were associated with the cell cycle and cell proliferation. To determine whether the observed patterns in cell-cycle gene expression in Clock mutants resulted in functional dysregulation, we compared proliferation rates of fibroblasts derived from WT or Clock mutant embryos and found that the Clock mutation significantly inhibits cell growth and proliferation.

Original languageEnglish (US)
Pages (from-to)3342-3347
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number9
DOIs
StatePublished - Feb 27 2007

Fingerprint

Transcriptome
Cell Proliferation
Gene Expression
Genes
Circadian Rhythm
Liver
Skeletal Muscle
cdc Genes
Cell Physiological Phenomena
Mutation
Oligonucleotide Array Sequence Analysis
Cocaine
Cell Cycle
Transcription Factors
Embryonic Structures
Obesity
Fibroblasts
Morbidity
Muscles
Growth

Keywords

  • Cell cycle
  • Circadian rhythm
  • Clock mutation
  • Gene expression
  • Protein-encoding transcriptome

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Circadian and CLOCK-controlled regulation of the mouse transcriptome and cell proliferation. / Miller, Brooke H.; McDearmon, Erin L.; Panda, Satchidananda; Hayes, Kevin R.; Zhang, Jie; Andrews, Jessica L.; Antoch, Marina P.; Walker, John R.; Esser, Karyn A.; Hogenesch, John B.; Takahashi, Joseph S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 9, 27.02.2007, p. 3342-3347.

Research output: Contribution to journalArticle

Miller, BH, McDearmon, EL, Panda, S, Hayes, KR, Zhang, J, Andrews, JL, Antoch, MP, Walker, JR, Esser, KA, Hogenesch, JB & Takahashi, JS 2007, 'Circadian and CLOCK-controlled regulation of the mouse transcriptome and cell proliferation', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 9, pp. 3342-3347. https://doi.org/10.1073/pnas.0611724104
Miller, Brooke H. ; McDearmon, Erin L. ; Panda, Satchidananda ; Hayes, Kevin R. ; Zhang, Jie ; Andrews, Jessica L. ; Antoch, Marina P. ; Walker, John R. ; Esser, Karyn A. ; Hogenesch, John B. ; Takahashi, Joseph S. / Circadian and CLOCK-controlled regulation of the mouse transcriptome and cell proliferation. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 9. pp. 3342-3347.
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