Circadian organization of the mammalian retina

Gou Xiang Ruan, Dao Qi Zhang, Tongrong Zhou, Shin Yamazaki, Douglas G. McMahon

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The mammalian retina contains an endogenous circadian pacemaker that broadly regulates retinal physiology and function, yet the cellular origin and organization of the mammalian retinal circadian clock remains unclear. Circadian clock neurons generate daily rhythms via cell-autonomous autoregulatory clock gene networks, and, thus, to localize circadian clock neurons within the mammalian retina, we have studied the cell type-specific expression of six core circadian clock genes in individual, identified mouse retinal neurons, as well as characterized the clock gene expression rhythms in photoreceptor degenerate rd mouse retinas. Individual photoreceptors, horizontal, bipolar, dopaminergic (DA) amacrines, catecholaminergic (CA) amacrines, and ganglion neurons were identified either by morphology or by a tyrosine hydroxylase (TH) promoter-driven red fluorescent protein (RFP) fluorescent reporter. Cells were collected, and their transcriptomes were subjected to multiplex single-cell RT-PCR for the core clock genes Period (Per) 1 and 2, Cryptochrome (Cry) 1 and 2, Clock, and Bmal1. Individual horizontal, bipolar, DA, CA, and ganglion neurons, but not photoreceptors, were found to coordinately express all six core clock genes, with the lowest proportion of putative clock cells in photoreceptors (0%) and the highest proportion in DA neurons (30%). In addition, clock gene rhythms were found to persist for >25 days in isolated, cultured rd mouse retinas in which photoreceptors had degenerated. Our results indicate that multiple types of retinal neurons are potential circadian clock neurons that express key elements of the circadian autoregulatory gene network and that the inner nuclear and ganglion cell layers of the mammalian retina contain functionally autonomous circadian clocks.

Original languageEnglish (US)
Pages (from-to)9703-9708
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number25
DOIs
StatePublished - Jun 20 2006

Fingerprint

Circadian Clocks
Retina
Neurons
Ganglia
Retinal Neurons
Gene Regulatory Networks
Genes
Cryptochromes
Photoreceptor Cells
Dopaminergic Neurons
Tyrosine 3-Monooxygenase
Transcriptome
Gene Expression
Polymerase Chain Reaction

Keywords

  • Circadian clock
  • Clock gene
  • Mouse retina
  • Photoreceptor
  • Real-time PCR

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Circadian organization of the mammalian retina. / Ruan, Gou Xiang; Zhang, Dao Qi; Zhou, Tongrong; Yamazaki, Shin; McMahon, Douglas G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 25, 20.06.2006, p. 9703-9708.

Research output: Contribution to journalArticle

Ruan, Gou Xiang ; Zhang, Dao Qi ; Zhou, Tongrong ; Yamazaki, Shin ; McMahon, Douglas G. / Circadian organization of the mammalian retina. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 25. pp. 9703-9708.
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