Circadian regulation of lodopsin gene expression in embryonic photoreceptors in retinal cell culture

Mary E. Pierce, Hooshmand Sheshberadaran, Zhang Zhe Zhang, Lyle E. Fox, Meredithe L. Applebury, Joseph S. Takahashi

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

A circadian clock regulates a number of diverse physiological functions in the vertebrate eye. In this study, we show that mRNA for the red-sensitive cone pigment, iodopsin, fluctuates with a circadian rhythm in chicken retina. Transcript levels increase in the late afternoon just prior to the time of cone disc shedding. Furthermore, iodopsin mRNA levels are regulated similarly by a circadian oscillator in primary cultures of dispersed embryonic chick retina. Nuclear run-on experiments show that the circadian regulation of iodopsin transcript abundance occurs at the level of gene transcription. Our results provide a demonstration of clock-regulated gene expression in a vertebrate preparation maintained in cell culture.

Original languageEnglish (US)
Pages (from-to)579-584
Number of pages6
JournalNeuron
Volume10
Issue number4
DOIs
StatePublished - 1993

Fingerprint

Vertebrate Photoreceptor Cells
Gene Expression Regulation
Cell Culture Techniques
Vertebrates
Retina
Messenger RNA
Circadian Clocks
Circadian Rhythm
Chickens
Gene Expression
Genes
iodopsin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Circadian regulation of lodopsin gene expression in embryonic photoreceptors in retinal cell culture. / Pierce, Mary E.; Sheshberadaran, Hooshmand; Zhe Zhang, Zhang; Fox, Lyle E.; Applebury, Meredithe L.; Takahashi, Joseph S.

In: Neuron, Vol. 10, No. 4, 1993, p. 579-584.

Research output: Contribution to journalArticle

Pierce, Mary E. ; Sheshberadaran, Hooshmand ; Zhe Zhang, Zhang ; Fox, Lyle E. ; Applebury, Meredithe L. ; Takahashi, Joseph S. / Circadian regulation of lodopsin gene expression in embryonic photoreceptors in retinal cell culture. In: Neuron. 1993 ; Vol. 10, No. 4. pp. 579-584.
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