Circular dichroism of isolated ricin A- and B-chains

Edward J. Wawrzynczak, Alex F. Drake, Philip E. Thorpe

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

An analysis of the circular dichroism (CD) spectra of isolated ricin A- and B-chains revealed several bands not apparent in the spectrum of intactricin. Arithmetic combination of the A- and B-chain spectra gave a composite spectrum resembling that of native ricin, indicating that the two chains did not undergo any major conformational change upon dissociation. The addition of lactose to the B-chain at pH 7.2 caused a slight perturbation of a tryptophan-derived negative CD band centred at 283 nm without change to the overall structure of the polypeptide.

Original languageEnglish (US)
Pages (from-to)301-305
Number of pages5
JournalBiophysical Chemistry
Volume31
Issue number3
DOIs
StatePublished - Sep 1988

Keywords

  • A-chain
  • B-chain
  • CD
  • Lactose binding
  • Ricin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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    Wawrzynczak, E. J., Drake, A. F., & Thorpe, P. E. (1988). Circular dichroism of isolated ricin A- and B-chains. Biophysical Chemistry, 31(3), 301-305. https://doi.org/10.1016/0301-4622(88)80036-X