Circular RNA 101368/miR-200a axis modulates the migration of hepatocellular carcinoma through HMGB1/RAGE signaling

Shaling Li, Huimin Gu, Yan Huang, Qian Peng, Rongrong Zhou, Panpan Yi, Ruochan Chen, Zebing Huang, Xingwang Hu, Yun Huang, Daolin Tang

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Hepatocellular carcinoma (HCC), one of the most common type of cancers, is highly refractory to most systemic therapies. Understanding the genomic dysregulations, in particularly non-coding RNA (ncRNA) dysregulations, in HCC may provide novel strategies to HCC treatment. In our previous study, we demonstrated the key role of miR-200a-mediated HMGB1/RAGE signaling in HCC carcinogenesis. In the present study, we identified circular RNA (circRNA)-miRNA pair that might modulate the migration of HCC cell lines based on previously reported GEO database (GSE78520 and GSE43445) and investigated the function and molecular mechanism. circRNA-101368 was predicted by lncTar to target miR-200a, and the expression of circRNA-101368 was significantly upregulated in HCC tissue samples; the overexpression of circRNA-101368 was correlated with poorer prognosis in patients with HCC. Moreover, circRNA-101368 knockdown suppressed the migration and the protein levels of HMGB1, RAGE and NF-κB, while increased the E-Cadherin expression in HCC cell lines. As confirmed by luciferase reporter and RNA immunoprecipitation assays, circRNA-101368 directly bound to miR-200a to negatively regulate each other. The effect of circRNA-101368 knockdown on cell migration and HMGB1/RAGE signaling could be partially attenuated by miR-200a inhibition. In tissue samples, miR-200a was negatively correlated with circRNA-101368 and HMGB1, respectively, whereas circRNA-101368 and HMGB1 was positively correlated. Taken together, we demonstrated a network of circRNAs-miRNA-mRNA in HCC and provided a novel mechanism of HCC cell migration regulation.

Original languageEnglish (US)
Pages (from-to)2349-2359
Number of pages11
JournalCell Cycle
Volume17
Issue number19-20
DOIs
StatePublished - Oct 18 2018
Externally publishedYes

Keywords

  • HMGB1
  • Hepatocellular carcinoma (HCC)
  • circRNA-101368

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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