Circulating xanthine oxidase mediates lung neutrophil sequestration after intestinal ischemia-reperfusion

L. S. Terada, J. J. Dormish, P. F. Shanley, J. A. Leff, B. O. Anderson, J. E. Repine

Research output: Contribution to journalArticlepeer-review

154 Scopus citations

Abstract

Injury to nonpulmonary organ systems often initiates systemic processes that cause recruitment of neutrophils to the lung. We found that rats subjected to intestinal ischemia-reperfusion (I/R) had increased transvascular leak of 125I-labeled albumin into lungs and decreased lung ATP levels (P < 0.05). In addition, rats subjected to intestinal I/R had increased plasma xanthine oxidase (XO) activity, plasma leukotactic activity for neutrophils, and lung neutrophil retention (assessed by morphometry and myeloperoxidase activity) compared with sham-treated rats (P < 0.05). By comparison, after intestinal I/R, rats fed an allopurinol- or tungsten- enriched diet had decreased plasma and intestinal XO activities, decreased plasma leukotactic and lung myeloperoxidase (MPO) activities, decreased lung leak, and increased lung ATP levels compared with rats fed control diets (P < 0.05). Further studies suggested a more specific role for circulating rather than tissue XO in mediating lung neutrophil accumulation but not lung leak. Plasma XO, plasma leukotactic, and lung MPO activities, but not lung leak, increased in rats administered purified XO intravenously. In addition, plasma XO, plasma leukotactic, and lung MPO activities, but not lung leak, decreased in rats administered antisera against XO and then subjected to intestinal I/R. We conclude that circulating XO increases acutely and may contribute to pulmonary retention of neutrophils after an ischemic intestinal insult.

Original languageEnglish (US)
Pages (from-to)L394-L401
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume263
Issue number3 7-3
DOIs
StatePublished - 1992

Keywords

  • adult respiratory distress syndrome
  • chemotaxis
  • inflammation
  • multiple organ failure
  • oxygen radicals
  • superoxide
  • vascular injury

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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