Cis-regulatory mechanisms of gene expression in an olfactory neuron type in Caenorhabditis elegans

Eva B. Nokes, Alexander M. Van Der Linden, Caron Winslow, Saikat Mukhopadhyay, Kristin Ma, Piali Sengupta

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The generation of cellular diversity is dependent on the precise spatiotemporal regulation of gene expression by both cis- and trans-acting mechanisms. The developmental principles regulating expression of specific gene subsets in individual cell types are not fully understood. Here we define the cis-regulatory mechanisms driving expression of cell-selective and broadly expressed genes in vivo in the AWB olfactory neuron subtype in C. elegans. We identify an element that is necessary to drive expression of neuron-selective chemoreceptor genes in the AWB neurons, and show that this element functions in a context-dependent manner. We find that the expression of broadly expressed sensory neuronal genes in the AWB neurons is regulated by diverse cis- and trans-regulatory mechanisms that act partly in parallel to the pathways governing expression of AWB-selective genes. We further demonstrate that cis-acting mechanisms driving gene expression in the AWB neurons appear to have diverged in related nematode species. Our results provide insights into the cis-regulatory logic driving cell-specific gene expression, and suggest that variations in this logic contribute to the generation of functional diversity.

Original languageEnglish (US)
Pages (from-to)3080-3092
Number of pages13
JournalDevelopmental Dynamics
Volume238
Issue number12
DOIs
StatePublished - Dec 2009

Fingerprint

Caenorhabditis elegans
Regulator Genes
Gene Expression
Neurons
Genes
Gene Expression Regulation

Keywords

  • Caenorhabditis elegans
  • Chemoreceptors
  • cis-regulatory motifs
  • Olfactory neuron

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Cis-regulatory mechanisms of gene expression in an olfactory neuron type in Caenorhabditis elegans. / Nokes, Eva B.; Van Der Linden, Alexander M.; Winslow, Caron; Mukhopadhyay, Saikat; Ma, Kristin; Sengupta, Piali.

In: Developmental Dynamics, Vol. 238, No. 12, 12.2009, p. 3080-3092.

Research output: Contribution to journalArticle

Nokes, Eva B. ; Van Der Linden, Alexander M. ; Winslow, Caron ; Mukhopadhyay, Saikat ; Ma, Kristin ; Sengupta, Piali. / Cis-regulatory mechanisms of gene expression in an olfactory neuron type in Caenorhabditis elegans. In: Developmental Dynamics. 2009 ; Vol. 238, No. 12. pp. 3080-3092.
@article{2a43f9ae310244b49be11b71722056af,
title = "Cis-regulatory mechanisms of gene expression in an olfactory neuron type in Caenorhabditis elegans",
abstract = "The generation of cellular diversity is dependent on the precise spatiotemporal regulation of gene expression by both cis- and trans-acting mechanisms. The developmental principles regulating expression of specific gene subsets in individual cell types are not fully understood. Here we define the cis-regulatory mechanisms driving expression of cell-selective and broadly expressed genes in vivo in the AWB olfactory neuron subtype in C. elegans. We identify an element that is necessary to drive expression of neuron-selective chemoreceptor genes in the AWB neurons, and show that this element functions in a context-dependent manner. We find that the expression of broadly expressed sensory neuronal genes in the AWB neurons is regulated by diverse cis- and trans-regulatory mechanisms that act partly in parallel to the pathways governing expression of AWB-selective genes. We further demonstrate that cis-acting mechanisms driving gene expression in the AWB neurons appear to have diverged in related nematode species. Our results provide insights into the cis-regulatory logic driving cell-specific gene expression, and suggest that variations in this logic contribute to the generation of functional diversity.",
keywords = "Caenorhabditis elegans, Chemoreceptors, cis-regulatory motifs, Olfactory neuron",
author = "Nokes, {Eva B.} and {Van Der Linden}, {Alexander M.} and Caron Winslow and Saikat Mukhopadhyay and Kristin Ma and Piali Sengupta",
year = "2009",
month = "12",
doi = "10.1002/dvdy.22147",
language = "English (US)",
volume = "238",
pages = "3080--3092",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "12",

}

TY - JOUR

T1 - Cis-regulatory mechanisms of gene expression in an olfactory neuron type in Caenorhabditis elegans

AU - Nokes, Eva B.

AU - Van Der Linden, Alexander M.

AU - Winslow, Caron

AU - Mukhopadhyay, Saikat

AU - Ma, Kristin

AU - Sengupta, Piali

PY - 2009/12

Y1 - 2009/12

N2 - The generation of cellular diversity is dependent on the precise spatiotemporal regulation of gene expression by both cis- and trans-acting mechanisms. The developmental principles regulating expression of specific gene subsets in individual cell types are not fully understood. Here we define the cis-regulatory mechanisms driving expression of cell-selective and broadly expressed genes in vivo in the AWB olfactory neuron subtype in C. elegans. We identify an element that is necessary to drive expression of neuron-selective chemoreceptor genes in the AWB neurons, and show that this element functions in a context-dependent manner. We find that the expression of broadly expressed sensory neuronal genes in the AWB neurons is regulated by diverse cis- and trans-regulatory mechanisms that act partly in parallel to the pathways governing expression of AWB-selective genes. We further demonstrate that cis-acting mechanisms driving gene expression in the AWB neurons appear to have diverged in related nematode species. Our results provide insights into the cis-regulatory logic driving cell-specific gene expression, and suggest that variations in this logic contribute to the generation of functional diversity.

AB - The generation of cellular diversity is dependent on the precise spatiotemporal regulation of gene expression by both cis- and trans-acting mechanisms. The developmental principles regulating expression of specific gene subsets in individual cell types are not fully understood. Here we define the cis-regulatory mechanisms driving expression of cell-selective and broadly expressed genes in vivo in the AWB olfactory neuron subtype in C. elegans. We identify an element that is necessary to drive expression of neuron-selective chemoreceptor genes in the AWB neurons, and show that this element functions in a context-dependent manner. We find that the expression of broadly expressed sensory neuronal genes in the AWB neurons is regulated by diverse cis- and trans-regulatory mechanisms that act partly in parallel to the pathways governing expression of AWB-selective genes. We further demonstrate that cis-acting mechanisms driving gene expression in the AWB neurons appear to have diverged in related nematode species. Our results provide insights into the cis-regulatory logic driving cell-specific gene expression, and suggest that variations in this logic contribute to the generation of functional diversity.

KW - Caenorhabditis elegans

KW - Chemoreceptors

KW - cis-regulatory motifs

KW - Olfactory neuron

UR - http://www.scopus.com/inward/record.url?scp=70949108185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70949108185&partnerID=8YFLogxK

U2 - 10.1002/dvdy.22147

DO - 10.1002/dvdy.22147

M3 - Article

C2 - 19924784

AN - SCOPUS:70949108185

VL - 238

SP - 3080

EP - 3092

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 12

ER -