Classification of the human phox homology (PX) domains based on their phosphoinositide binding specificities

Mintu Chandra, Yanni K.Y. Chin, Caroline Mas, J. Ryan Feathers, Blessy Paul, Sanchari Datta, Kai En Chen, Xinying Jia, Zhe Yang, Suzanne J. Norwood, Biswaranjan Mohanty, Andrea Bugarcic, Rohan D. Teasdale, William M Henne, Mehdi Mobli, Brett M. Collins

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Phox homology (PX) domains are membrane interacting domains that bind to phosphatidylinositol phospholipids or phosphoinositides, markers of organelle identity in the endocytic system. Although many PX domains bind the canonical endosome-enriched lipid PtdIns3P, others interact with alternative phosphoinositides, and a precise understanding of how these specificities arise has remained elusive. Here we systematically screen all human PX domains for their phospholipid preferences using liposome binding assays, biolayer interferometry and isothermal titration calorimetry. These analyses define four distinct classes of human PX domains that either bind specifically to PtdIns3P, non-specifically to various di- and tri-phosphorylated phosphoinositides, bind both PtdIns3P and other phosphoinositides, or associate with none of the lipids tested. A comprehensive evaluation of PX domain structures reveals two distinct binding sites that explain these specificities, providing a basis for defining and predicting the functional membrane interactions of the entire PX domain protein family.

Original languageEnglish (US)
Article number1528
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

    Fingerprint

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Chandra, M., Chin, Y. K. Y., Mas, C., Feathers, J. R., Paul, B., Datta, S., Chen, K. E., Jia, X., Yang, Z., Norwood, S. J., Mohanty, B., Bugarcic, A., Teasdale, R. D., Henne, W. M., Mobli, M., & Collins, B. M. (2019). Classification of the human phox homology (PX) domains based on their phosphoinositide binding specificities. Nature communications, 10(1), [1528]. https://doi.org/10.1038/s41467-019-09355-y