Clathrin-coated vesicle formation from isolated plasma membranes

Ishido Miwako; Sandra L. Schmid

doi:10.1016/S0076-6879(05)04044-9

Clathrin-coated vesicle formation from isolated plasma membranes

Ishido Miwako, Sandra L. Schmid

Research output: Contribution to journal › Review article › peer-review

6 Scopus citations

Abstract

Endocytic clathrin-coated vesicle (CCV) formation is a complex process involving a large number of proteins and lipids. The minimum machinery and the hierarchy of the events involved in CCV formation have yet to be defined. Here we describe an in vitro assay for CCV formation from highly purified rat liver plasma membranes. This rapid and easy assay can be used to quantitatively evaluate the different protein requirements for different endocytic receptors.

Original language	English (US)
Article number	44
Pages (from-to)	503-511
Number of pages	9
Journal	Methods in Enzymology
Volume	404
DOIs	https://doi.org/10.1016/S0076-6879(05)04044-9
State	Published - Jan 1 2005

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/S0076-6879(05)04044-9

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title = "Clathrin-coated vesicle formation from isolated plasma membranes",

abstract = "Endocytic clathrin-coated vesicle (CCV) formation is a complex process involving a large number of proteins and lipids. The minimum machinery and the hierarchy of the events involved in CCV formation have yet to be defined. Here we describe an in vitro assay for CCV formation from highly purified rat liver plasma membranes. This rapid and easy assay can be used to quantitatively evaluate the different protein requirements for different endocytic receptors.",

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AU - Schmid, Sandra L.

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AB - Endocytic clathrin-coated vesicle (CCV) formation is a complex process involving a large number of proteins and lipids. The minimum machinery and the hierarchy of the events involved in CCV formation have yet to be defined. Here we describe an in vitro assay for CCV formation from highly purified rat liver plasma membranes. This rapid and easy assay can be used to quantitatively evaluate the different protein requirements for different endocytic receptors.

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U2 - 10.1016/S0076-6879(05)04044-9

DO - 10.1016/S0076-6879(05)04044-9

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EP - 511

JO - Methods in Enzymology

JF - Methods in Enzymology

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Clathrin-coated vesicle formation from isolated plasma membranes

Abstract

ASJC Scopus subject areas

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