Cleavage site of the poliovirus receptor signal sequence

J. A. Bibb, G. Bernhardt, E. Wimmer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have shown recently that the human poliovirus receptors (hPVRs) expressed on the surface of cultured cells are 80K glycoproteins, whereas the previously reported 67K forms are partially glycosylated intermediate glycoforms. Both the membrane-bound 80K and 67K forms of hPVR are glycosylated derivatives of the two isoforms hPVRα and hPVRδ, where the latter two can be resolved only by SDS-PAGE upon enzymatic deglycosylation. Here we report the N-terminal sequence analysis of the mature 80K as well as the intermediate 67K glycoforms of hPVR which has allowed us to identify the signal peptidase cleavage site of the unprocessed hPVR. The signal sequence that directs translocation of hPVR across the membrane of the endoplasmic reticulum on its route to the glycoprocessing pathway has thus been defined. We compare this signal sequence with those of the putative monkey poliovirus receptor and the mouse poliovirus receptor homologue.

Original languageEnglish (US)
Pages (from-to)1875-1881
Number of pages7
JournalJournal of General Virology
Volume75
Issue number8
StatePublished - 1994

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Protein Sorting Signals
Theilovirus
poliovirus receptor
Membranes
Endoplasmic Reticulum
Haplorhini
Sequence Analysis
Polyacrylamide Gel Electrophoresis
Cultured Cells
Glycoproteins
Protein Isoforms

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Cleavage site of the poliovirus receptor signal sequence. / Bibb, J. A.; Bernhardt, G.; Wimmer, E.

In: Journal of General Virology, Vol. 75, No. 8, 1994, p. 1875-1881.

Research output: Contribution to journalArticle

Bibb, JA, Bernhardt, G & Wimmer, E 1994, 'Cleavage site of the poliovirus receptor signal sequence', Journal of General Virology, vol. 75, no. 8, pp. 1875-1881.
Bibb, J. A. ; Bernhardt, G. ; Wimmer, E. / Cleavage site of the poliovirus receptor signal sequence. In: Journal of General Virology. 1994 ; Vol. 75, No. 8. pp. 1875-1881.
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