Climbing fiber-Purkinje cell synaptic pathology in tremor and cerebellar degenerative diseases

Sheng Han Kuo, Chi Ying Lin, Jie Wang, Peter A. Sims, Ming Kai Pan, Jyun you Liou, Danielle Lee, William J. Tate, Geoffrey C. Kelly, Elan D. Louis, Phyllis L. Faust

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Changes in climbing fiber-Purkinje cell (CF-PC) synaptic connections have been found in the essential tremor (ET) cerebellum, and these changes are correlated with tremor severity. Whether these postmortem changes are specific to ET remains to be investigated. We assessed CF-PC synaptic pathology in the postmortem cerebellum across a range of degenerative movement disorders [10 Parkinson’s disease (PD) cases, 10 multiple system atrophy (MSA) cases, 10 spinocerebellar ataxia type 1 (SCA1) cases, and 20 ET cases] and 25 controls. We observed differences in terms of CF pathological features across these disorders. Specifically, PD cases and ET cases both had more CFs extending into the parallel fiber (PF) territory, but ET cases had more complex branching and increased length of CFs in the PF territory along with decreased CF synaptic density compared to PD cases. MSA cases and SCA1 cases had the most severely reduced CF synaptic density and a marked paucity of CFs extending into the PF territory. Furthermore, CFs in a subset of MSA cases formed collateral branches parallel to the PC layer, a feature not seen in other diagnostic groups. Using unsupervised cluster analysis, the cases and controls could all be categorized into four clusters based on the CF pathology and features of PC pathology, including counts of PCs and their axonal torpedoes. ET cases and PD cases co-segregated into two clusters, whereas SCA1 cases and MSA cases formed another cluster, separate from the control cluster. Interestingly, the presence of resting tremor seemed to be the clinical feature that separated the cases into the two ET-PD clusters. In conclusion, our study demonstrates that these degenerative movement disorders seem to differ with respect to the pattern of CF synaptic pathology they exhibit. It remains to be determined how these differences contribute to the clinical presentations of these diseases.

Original languageEnglish (US)
Pages (from-to)121-138
Number of pages18
JournalActa Neuropathologica
Volume133
Issue number1
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Keywords

  • Climbing fiber
  • Essential tremor
  • Multiple system atrophy
  • Parkinson’s disease
  • Purkinje cell
  • Spinocerebellar ataxia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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    Kuo, S. H., Lin, C. Y., Wang, J., Sims, P. A., Pan, M. K., Liou, J. Y., Lee, D., Tate, W. J., Kelly, G. C., Louis, E. D., & Faust, P. L. (2017). Climbing fiber-Purkinje cell synaptic pathology in tremor and cerebellar degenerative diseases. Acta Neuropathologica, 133(1), 121-138. https://doi.org/10.1007/s00401-016-1626-1