Clinical and diagnostic use of in vivo confocal microscopy in patients with corneal disease

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256 Citations (Scopus)

Abstract

Background: The purpose of this article is to introduce the practicing ophthalmologist to the optical principles and images produced by a tandem scanning confocal microscope (recently approved by the Food and Drug Administration for general clinical use). The tandem scanning confocal microscope allows real-time viewing of structures in the living cornea at the cellular level in four dimensions (x, y, z, and time). Methods: Nine patients (2 males, 7 females), ranging in age from 7 to 52 years, were examined. Images were recorded on super VHS videotape, digitized and processed on a computer workstation, and photographed for presentation. Results: Two- dimensional (x, y) 400 x 400-μm images (9-μm z-axis thickness) are presented for normal corneal structures and for the clinical conditions of herpetic keratitis, wound healing after myopic excimer ablation, Acanthamoeba infection, corneal dystrophies (granular, Reis-Buckler), contact lens abrasion, and the irido-corneal endothelial syndrome. Conclusion: Clinical confocal microscopy has the unique potential of providing noninvasive assessment of corneal injury and disease at the cellular level that is not available currently from other technologies.

Original languageEnglish (US)
Pages (from-to)1444-1454
Number of pages11
JournalOphthalmology
Volume100
Issue number10
StatePublished - 1993

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Corneal Diseases
Confocal Microscopy
Herpetic Keratitis
Acanthamoeba
Videotape Recording
Contact Lenses
United States Food and Drug Administration
Wound Healing
Cornea
Technology
Infection
Intravital Microscopy
Ophthalmologists
Groenouw type I corneal dystrophy
Corneal Injuries

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Clinical and diagnostic use of in vivo confocal microscopy in patients with corneal disease. / Cavanagh, Harrison D; Petroll, Walter M; Alizadeh, H.; He, Yu-Guang; McCulley, James P; Jester, J. V.

In: Ophthalmology, Vol. 100, No. 10, 1993, p. 1444-1454.

Research output: Contribution to journalArticle

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AU - McCulley, James P

AU - Jester, J. V.

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